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Titolo:
Transport of choline and its relationship to the expression of the organiccation transporters in a rat brain microvessel endothelial cell line (RBE4)
Autore:
Friedrich, A; George, RL; Bridges, CC; Prasad, PD; Ganapathy, V;
Indirizzi:
Med Coll Georgia, Dept Biochem & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 m & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia, Dept Obstet & Gynecol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 et & Gynecol, Augusta, GA 30912 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
fascicolo: 2, volume: 1512, anno: 2001,
pagine: 299 - 307
SICI:
0005-2736(20010606)1512:2<299:TOCAIR>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-MEMBRANE VESICLES; FUNCTIONAL EXPRESSION; BARRIER TRANSPORT; NERVOUS-SYSTEM; CLONING; OCT3; IDENTIFICATION; NEURONS; CULTURE; KIDNEY;
Keywords:
choline uptake; hemicholinium-3; organic cation transporter; blood-brain barrier; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Ganapathy, V Med Coll Georgia, Dept Biochem & Mol Biol, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 Augusta, GA 30912 USA
Citazione:
A. Friedrich et al., "Transport of choline and its relationship to the expression of the organiccation transporters in a rat brain microvessel endothelial cell line (RBE4)", BBA-BIOMEMB, 1512(2), 2001, pp. 299-307

Abstract

present study was undertaken to elucidate the functional characteristics of choline uptake and deduce the relationship between choline uptake and theexpression of organic cation transporters in the rat brain microvessel endothelial cell line RBE4. Confluent RBE4 cells were found to express a high affinity choline uptake system. The system is Na+-independent and shows a Michaelis-Menten constant of approx. 20 muM for choline. The choline analogue hemicholinium-3 inhibits choline uptake in these cells with an inhibitionconstant of approx. 50 muM The uptake system is also susceptible for inhibition by various organic cations, including 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, clonidine, procainamide, and tetramethylammonium. The prototypical organic cation tetraethylammonium showsvery little affinity for the choline uptake system in these cells. The inhibition of choline uptake by hemicholinium-3 is competitive. Northern analysis and RT-PCR show that these cells do not express the organic cation transporters OCT2 and OCT3. These cells do express, however, low levels of OCT1, but the functional characteristics of choline uptake in these cells are very different from the known properties of choline uptake via OCT1. The Na+-coupled high affinity choline transporter CHT1 is not expressed in these cells as evidenced by RT-PCR. This corroborates the Na+-independent nature of choline uptake in these cells. It is concluded that RBE4 cells express anorganic cation transporter that is responsible for choline uptake in thesecells and that this transporter is not identical to any of the organic cation transporters thus far identified at the molecular level in mammalian cells. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:50:35