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Titolo:
A mitochondrial uncoupling artifact can be caused by expression of uncoupling protein 1 in yeast
Autore:
Stuart, JA; Harper, JA; Brindle, KM; Jekabsons, MB; Brand, MD;
Indirizzi:
MRC, Dunn Human Nutr Unit, Cambridge CB2 2XY, England MRC Cambridge England CB2 2XY uman Nutr Unit, Cambridge CB2 2XY, England Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England Univ Cambridge Cambridge England CB2 1GA hem, Cambridge CB2 1GA, England
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 356, anno: 2001,
parte:, 3
pagine: 779 - 789
SICI:
0264-6021(20010615)356:<779:AMUACB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
BROWN-ADIPOSE-TISSUE; ELECTROCHEMICAL POTENTIAL GRADIENT; EFFECTIVE PROTON CONDUCTANCE; RAT SKELETAL-MUSCLE; INNER MEMBRANE; MESSENGER-RNA; NUCLEOTIDE-BINDING; TRANSPORT; THERMOGENESIS; MICE;
Keywords:
mitochondria; proton leak; uncoupling protein 1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Brand, MD MRC, Dunn Human Nutr Unit, Hills Rd, Cambridge CB2 2XY, England MRC Hills Rd Cambridge England CB2 2XY mbridge CB2 2XY, England
Citazione:
J.A. Stuart et al., "A mitochondrial uncoupling artifact can be caused by expression of uncoupling protein 1 in yeast", BIOCHEM J, 356, 2001, pp. 779-789

Abstract

Uncoupling protein 1 (UCP1) from mouse was expressed in yeast and the specific (GDP-inhibitable) and artifactual (GDP-insensitive) effects on mitochondrial uncoupling were assessed. UCP 1 provides a GDP-inhibitable model system to help interpret the uncoupling effects of high expression in yeast ofother members of the mitochondrial carrier protein family, such as the UCP1 homologues UCP2 and UCP3. Yeast expressing UCP1 at modest levels (approx.1 mug/mg of mitochondrial protein) showed no growth defect, normal rates of chemically uncoupled respiration and an increased non-phosphorylating proton conductance that was completely GDP-sensitive. The catalytic-centre activity of UCP1 in these yeast mitochondria was similar to that in mammalian brown-adipose-tissue mitochondria. However, yeast expressing UCP1 at higherlevels (approx. 11 mug/mg of mitochondrial protein) showed a growth defect. Their mitochondria had depressed chemically uncoupled respiration rates and an increased proton conductance that was partly GDP-insensitive. Thus, although UCP1 shows native behaviour at modest levels of expression in yeast, higher levels (or rates) of expression can lead to an uncoupling that is not a physiological property of the native protein and is therefore artifactual. This observation might be important in the interpretation of results from experiments in which the functions of UCP1 homologues are verified by their ability to uncouple yeast mitochondria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:47:44