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Titolo:
Conserved base-pairings between C266-A268 and U307-G309 in the P7 of the Tetrahymena ribozyme is nonessential for the in vitro self-splicing reaction
Autore:
Oe, Y; Ikawa, Y; Shiraishi, H; Inoue, T;
Indirizzi:
Kyoto Univ, Grad Sch Sci, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Univ, Grad Sch Sci, Kyoto 6068502, Japan Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Grad Sch Biostudies, Kyoto 6068502, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 4, volume: 284, anno: 2001,
pagine: 948 - 954
SICI:
0006-291X(20010622)284:4<948:CBBCAU>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; BINDING-SITE; RNA; PRECURSOR;
Keywords:
ribozyme; group I intron; self-splicing; Tetrahymena; P7;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, T Kyoto Univ, Grad Sch Sci, Kyoto 6068502, Japan Kyoto Univ KyotoJapan 6068502 ad Sch Sci, Kyoto 6068502, Japan
Citazione:
Y. Oe et al., "Conserved base-pairings between C266-A268 and U307-G309 in the P7 of the Tetrahymena ribozyme is nonessential for the in vitro self-splicing reaction", BIOC BIOP R, 284(4), 2001, pp. 948-954

Abstract

P7 is highly conserved in Group I self-splicing intron ribozymes. This region is known to coordinate metal ions and bind a cofactor guanosine required for the self-splicing, To further investigate the fundamental role of thecorresponding region in the Tetrahymena ribozyme, we attempted to identifyminimal requirements for the base-paired region excluding the guanosine binding site. We discovered that a variety of sequences are eligible and its derivatives possessing extra nucleotide(s) can still conduct the first stepof splicing, indicating that no particular base-pairing is essential in this region for conducting the reaction in vitro. The results provide two hypotheses for the fundamental role of this region: (i) if the region containselement(s) that are strictly required in the catalysis, they are not necessarily tightly fixed in the ribozyme and (ii) if not, its fundamental role may simply be to coordinate neighboring regions that are directly involved in the catalysis. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/02/20 alle ore 05:05:28