Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
2 MURINE HOMOLOGS OF THE HUMAN CHEMOKINE RECEPTOR CXCR4 MEDIATING STROMAL CELL-DERIVED FACTOR 1-ALPHA ACTIVATION OF G(12) ARE DIFFERENTIALLY EXPRESSED IN-VIVO
Autore:
MOEPPS B; FRODL R; RODEWALD HR; BAGGIOLINI M; GIERSCHIK P;
Indirizzi:
UNIV ULM,DEPT PHARMACOL & TOXICOL,ALBERT EINSTEIN ALLEE 11 D-89081 ULM GERMANY UNIV ULM,DEPT PHARMACOL & TOXICOL D-89081 ULM GERMANY BASEL INST IMMUNOL BASEL SWITZERLAND UNIV BERN,THEODOR KOCHER INST BERN SWITZERLAND
Titolo Testata:
European Journal of Immunology
fascicolo: 8, volume: 27, anno: 1997,
pagine: 2102 - 2112
SICI:
0014-2980(1997)27:8<2102:2MHOTH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN C5A RECEPTOR; INTERLEUKIN-8 RECEPTOR; MOLECULAR-CLONING; PERTUSSIS TOXIN; NEUROPEPTIDE-Y; AMINO-TERMINUS; CHROMOSOMAL LOCALIZATION; NPY BINDING; GENE; PROTEINS;
Keywords:
CHEMOKINE RECEPTOR; THYMOCYTE; G PROTEIN; AIDS; HIV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
B. Moepps et al., "2 MURINE HOMOLOGS OF THE HUMAN CHEMOKINE RECEPTOR CXCR4 MEDIATING STROMAL CELL-DERIVED FACTOR 1-ALPHA ACTIVATION OF G(12) ARE DIFFERENTIALLY EXPRESSED IN-VIVO", European Journal of Immunology, 27(8), 1997, pp. 2102-2112

Abstract

Previous results have shown that pertussis toxin-sensitive G(i) proteins are likely to be involved in regulating the emigration of mature thymocytes from the thymus. In this study, a low stringency polymerase chain reaction (PCR) approach was used to identify G(i) protein-coupled cell surface receptors expressed in mouse thymocytes. Among the ten G protein-coupled receptor cDNA isolated, the most prevalent cDNA encoded a polypeptide highly homologous to the human leukocyte-expressed seven-transmembrane-domain receptor LESTR, also referred to as HIV entry cofactor, fusin, or CXCR4. Isolation of full-length cDNA revealed that alternative RNA splicing produces transcripts encoding two isoformsof the murine LESTR, differing by the presence of two amino acids in the N-terminal portion of the longer protein. Functional reconstitution of recombinant murine LESTR with recombinant heterotrimeric G proteins in baculovirus-infected insect cells showed that both receptor variants mediate stromal cell-derived factor 1 alpha activation of the pertussis toxin-sensitive G protein G(i2). Receptor subtype-specific reverse transcriptase-PCR analysis revealed differential expression of thetwo receptor mRNA in lymphoid tissues and brain, indicating that distinct functions are mediated by the two receptor isoforms in these tissues. The presence of LESTR mRNA in very early thymocytes as well as inimmature (CD4(+) CD8(+)) thymocytes suggests that both CD4 and LESTR are co-expressed and render developing human thymocytes susceptible for HIV entry which may affect generation of both CD4(+) CD8(-) and CD4(-) CD8(+) mature lineages.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:42:31