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Titolo:
REDUCED INTRACELLULAR OXIDATIVE-METABOLISM PROMOTES FIRM ADHESION OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES TO VASCULAR ENDOTHELIUM UNDER FLOW CONDITIONS
Autore:
MONTOYA MC; LUSCINSKAS FW; DELPOZO MA; ARAGONES J; DELANDAZURI MO;
Indirizzi:
UNIV AUTONOMA MADRID,HOSP PRINCESA,SERV INMUNOL,C DIEGO DE LEON 62 E-28006 MADRID SPAIN UNIV AUTONOMA MADRID,HOSP PRINCESA,SERV INMUNOL E-28006 MADRID SPAIN HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT PATHOL BOSTON MA 02115
Titolo Testata:
European Journal of Immunology
fascicolo: 8, volume: 27, anno: 1997,
pagine: 1942 - 1951
SICI:
0014-2980(1997)27:8<1942:RIOPFA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; HUMAN-NEUTROPHILS; CHEMOTACTIC FACTORS; TRANSIENT ADHESION; LIPID-PEROXIDATION; SURFACE EXPRESSION; SIGNALING PATHWAY; CD11B/CD18 MAC-1; CELL-ADHESION; PHORBOL ESTER;
Keywords:
NEUTROPHIL; HYPOXIA; ANTIOXIDANT; ADHESION; FLOW;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Montoya et al., "REDUCED INTRACELLULAR OXIDATIVE-METABOLISM PROMOTES FIRM ADHESION OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES TO VASCULAR ENDOTHELIUM UNDER FLOW CONDITIONS", European Journal of Immunology, 27(8), 1997, pp. 1942-1951

Abstract

The interaction of polymorphonuclear leukocytes (PMN) with the vascular endothelium and their subsequent extravasation to the tissues is a key step during different physiological and pathological processes. Incertain of these pathologies the oxygen tension becomes very low, leading to reduced cellular oxidative status. To evaluate the effect of lowering the intracellular redox status in the interaction of PMN with the endothelium, exposure to hypoxic conditions as well as treatment with different antioxidant agents was carried out. PMN exposure to hypoxia enhanced beta(2) integrin-dependent adhesion to intercellular adhesion molecule-1-coated surfaces, concomitant with a decrease in the intracellular redox status of the cell. As occurs with hypoxia, treatment with antioxidants produced a decrease in the oxidation state of PMN. These agents enhanced adhesion of PMN to human umbilical vein endothelial cells stimulated with tumor necrosis factor-alpha (TNF-alpha), and this effect was also mediated by beta(2) integrins LFA-1 and Mac-1. Adhesion studies under defined laminar flow conditions showed that theantioxidant treatment induced an enhanced adhesion mediated by beta(2) integrins with a decrease in the fraction of PMN rolling on TNF-alpha-activated endothelial cells. The up-regulated PMN adhesion was correlated to an increase in the expression and activation of integrin Mac-1, without loss of L-selectin surface expression. Altogether these results demonstrate that a reduction in the intracellular oxidative stateproduces an enhanced beta(2) integrin-dependent adhesion of PMN to stimulated endothelial cells under conditions of flow.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 21:51:05