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Titolo:
alpha(1)-Adrenoceptor subtypes on rat afferent arterioles assessed by radioligand binding and RT-PCR
Autore:
Salomonsson, M; Oker, M; Kim, S; Zhang, H; Faber, JE; Arendshorst, WJ;
Indirizzi:
Univ N Carolina, Sch Med, Dept Cell & Mol Physiol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 ysiol, Chapel Hill, NC 27599 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 1, volume: 281, anno: 2001,
pagine: F172 - F178
SICI:
0363-6127(200107)281:1<F172:ASORAA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUSLY HYPERTENSIVE RATS; VASCULAR SMOOTH-MUSCLE; ALPHA-1-ADRENOCEPTOR SUBTYPES; ALPHA-ADRENOCEPTORS; ADRENERGIC-RECEPTORS; MESSENGER-RNA; RENAL-ARTERY; KIDNEY; ALPHA(1A)-ADRENOCEPTOR; VASOCONSTRICTION;
Keywords:
renal circulation; vascular smooth muscle; reverse transcription-polymerase chain reaction; mRNA; renal nerve; catecholamine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Arendshorst, WJ Univ N Carolina, Sch Med, Dept Cell & Mol Physiol, CB 7545,Rm 152,Med Sci Res Bldg, Chapel Hill, NC 27599 USA Univ N Carolina CB 7545,Rm 152,Med Sci Res Bldg Chapel Hill NC USA 27599
Citazione:
M. Salomonsson et al., "alpha(1)-Adrenoceptor subtypes on rat afferent arterioles assessed by radioligand binding and RT-PCR", AM J P-REN, 281(1), 2001, pp. F172-F178

Abstract

We utilized [H-3]prazosin saturation and competition radioligand binding studies to characterize the expression of alpha (1)-adrenoceptors in preglomerular vessels. mRNA for adrenoceptor subtypes was assayed using RT-PCR. The vessels were isolated using an iron oxide-sieving method. [H-3]prazosin bound to a single class of binding sites (K-d 0.087 +/- 0.012 nM, B-max 326 /- 56 fmol/mg protein). Phentolamine displaced [H-3]prazosin (0.2 nM) witha pK(i) of 8.37 +/- 0.09. Competition with the selective alpha (1A)- adrenoceptor antagonist 5-methylurapidil fit a two-site model (pK(i) 9.38 +/- 0.21 and 7.94 +/- 0.15); 59 +/- 3% of the sites were high-affinity, and 41 +/- 3% were low-affinity binding sites. Competition with the alpha (1D)-adrenoceptor antagonist 8-(2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl)-8-azaspiro[4.5] decane-7,9-dione dihydrochloride (BMY-7378) fit a one-site model with low affinity (pK(i) 6.83 +/- 0.03). The relative contents of alpha (1A)- alpha (1B)-, and alpha (1D)-adrenoceptor mRNAs were 64 +/- 5, 25 +/- 5, and11 +/- 1%, respectively. Thus there was a very good correlation between mRNA and receptor binding for the subtypes. These data indicate a predominance of the alpha (1A)-adrenoceptor subtype in rat renal resistance vessels, with smaller densities of alpha (1B)- and alpha (1D)-adrenoceptors.

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Documento generato il 01/12/20 alle ore 01:04:34