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Titolo:
Sarcoplasmic reticulum Ca2+ uptake is impaired in coronary smooth muscle distal to coronary occlusion
Autore:
Heaps, CL; Sturek, M; Price, EM; Laughlin, MH; Parker, JL;
Indirizzi:
Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 ovasc Res Ctr, Columbia, MO 65211 USA Univ Missouri, Dept Physiol, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Dept Physiol, Columbia, MO 65211 USA Univ Missouri, Dept Vet Biomed Sci, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 et Biomed Sci, Columbia, MO 65211 USA Univ Missouri, Dept Internal Med, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Internal Med, Columbia, MO 65211 USA Texas A&M Univ, Dept Med Physiol, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 hysiol, College Stn, TX 77843 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 1, volume: 281, anno: 2001,
pagine: H223 - H231
SICI:
0363-6135(200107)281:1<H223:SRCUII>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLATERAL CIRCULATION; ENDOTHELIAL MODULATION; ARTERY OCCLUSION; BARIUM INFLUX; CALCIUM; EXERCISE; CELLS; MICROCIRCULATION; SELECTIVITY; ISCHEMIA;
Keywords:
vascular smooth muscle; calcium; SERCA; exercise training; porcine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Heaps, CL Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USAUniv Missouri Columbia MO USA 65211 Ctr, Columbia, MO 65211 USA
Citazione:
C.L. Heaps et al., "Sarcoplasmic reticulum Ca2+ uptake is impaired in coronary smooth muscle distal to coronary occlusion", AM J P-HEAR, 281(1), 2001, pp. H223-H231

Abstract

After chronic occlusion, collateral-dependent coronary arteries exhibit alterations in both vasomotor reactivity and associated myoplasmic free Ca2+ levels that are prevented by chronic exercise training. We tested the hypotheses that coronary occlusion diminishes Ca2+ uptake by the sarcoplasmic reticulum (SR) and that exercise training would prevent impaired SR Ca2+ uptake. Ameroid constrictors were surgically placed around the proximal left circumflex (LCx) artery of female swine 8 wk before initiating 16-wk sedentary (pen confined) or exercise-training (treadmill run) protocols. Twenty-four weeks after Ameroid placement, smooth muscles cells were enzymatically dissociated from both the LCx and nonoccluded left anterior descending (LAD) arteries of sedentary and exercise-trained pigs, and myoplasmic free Ca2+ was studied using fura 2 microfluorometry. After the SR Ca2+ store was partially depleted with caffeine (5 mM), KCl-induced membrane depolarization produced a significant decrease in the time to half-maximal (t(1/2)) myoplasmic free Ca2+ accumulation in LCx versus LAD cells of sedentary pigs. Furthermore, inhibition of sarco (endo) plasmic reticulum Ca2+-ATPase (SERCA; 10 muM cyclopiazonic acid) significantly reduced t(1/2) in cells isolated from the LAD but not from the LCx. Exercise training did not prevent the differences in t(1/2) myoplasmic free Ca2+ accumulation observed between LCx and LAD cells. Occlusion or exercise training did not alter SERCA protein levels. These results support our hypothesis of impaired SR Ca2+ uptake in coronary smooth muscle cells isolated distal to chronic occlusion. Impaired SR Ca2+ uptake was independent of SERCA protein levels and was not prevented by exercise training.

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Documento generato il 30/09/20 alle ore 02:31:04