Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of a domain peptide of the ryanodine receptor on Ca2+ release in skinned skeletal muscle fibers
Autore:
Lamb, GD; Posterino, GS; Yamamoto, T; Ikemoto, N;
Indirizzi:
La Trobe Univ, Dept Zool, Bundoora, Vic 3086, Australia La Trobe Univ Bundoora Vic Australia 3086 , Bundoora, Vic 3086, Australia Boston Biomed Res Inst, Watertown, MA 02472 USA Boston Biomed Res Inst Watertown MA USA 02472 st, Watertown, MA 02472 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 1, volume: 281, anno: 2001,
pagine: C207 - C214
SICI:
0363-6143(200107)281:1<C207:EOADPO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-MALIGNANT HYPERTHERMIA; INDUCED FORCE RESPONSES; CALCIUM-RELEASE; SARCOPLASMIC-RETICULUM; VOLTAGE-DEPENDENCE; EXCITATION; RAT; TOAD; MG2+; CHANNELS;
Keywords:
excitation-contraction coupling; voltage sensor; action potential; malignant hyperthermia; caffeine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Lamb, GD La Trobe Univ, Dept Zool, Bundoora, Vic 3086, Australia La Trobe Univ Bundoora Vic Australia 3086 a, Vic 3086, Australia
Citazione:
G.D. Lamb et al., "Effects of a domain peptide of the ryanodine receptor on Ca2+ release in skinned skeletal muscle fibers", AM J P-CELL, 281(1), 2001, pp. C207-C214

Abstract

Mutations in the central domain of the skeletal muscle ryanodine receptor (RyR) cause malignant hyperthermia (MH). A synthetic peptide (DP4) in this domain (Leu-2442-Pro-2477) produces enhanced ryanodine binding and sensitized Ca2+ release in isolated sarcoplasmic reticulum, similar to the properties in MH, possibly because the peptide disrupts the normal interdomain interactions that stabilize the closed state of the RyR (Yamamoto T, El-Hayek R, and Ikemoto N. J Biol Chem 275: 11618-11625, 2000). Here, DP4 was appliedto mechanically skinned fibers of rat muscle that had the normal excitation-contraction coupling mechanism still functional to determine whether muscle fiber responsiveness was enhanced. DP4 (100 muM) substantially potentiated the Ca2+ release and force response to caffeine (8 mM) and to low [Mg2+](0.2 mM) in every fiber examined, with no significant effect on the properties of the contractile apparatus. DP4 also potentiated the response to submaximal depolarization of the transverse tubular system by ionic substitution. Importantly, DP4 did not significantly alter the size of the twitch response elicited by action potential stimulation. These results support the proposal that DP4 causes an MH-like aberration in RyR function and are consistent with the voltage sensor triggering Ca2+ release by destabilizing the closed state of the RyRs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:51:08