Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Phosphatidylinositol 3-kinase-dependent stabilization of alpha(1)(I) collagen mRNA in human lung fibroblasts
Autore:
Ricupero, DA; Poliks, CF; Rishikof, DC; Cuttle, KA; Kuang, PP; Goldstein, RH;
Indirizzi:
Boston Univ, Sch Med, Ctr Pulm, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 , Sch Med, Ctr Pulm, Boston, MA 02118 USA Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 h Med, Dept Biochem, Boston, MA 02118 USA Boston Vet Med Ctr, Boston, MA 02118 USA Boston Vet Med Ctr Boston MA USA02118 Vet Med Ctr, Boston, MA 02118 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 1, volume: 281, anno: 2001,
pagine: C99 - C105
SICI:
0363-6143(200107)281:1<C99:P3SOAC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ALPHA-1(I) MESSENGER-RNA; HUMAN MESANGIAL CELLS; ACTIVATES MAP KINASE; GROWTH FACTOR-BETA; PRO-ALPHA-1(I) COLLAGEN; INSULIN STIMULATION; PULMONARY FIBROSIS; MOUSE FIBROBLASTS; EXPRESSION;
Keywords:
mRNA stability; LY-294002; wortmannin; actinomycin D;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ricupero, DA Boston Univ, Sch Med, Ctr Pulm, Rm 304,80 E Concord St, Boston, MA 02118 USA Boston Univ Rm 304,80 E Concord St Boston MA USA 02118 18 USA
Citazione:
D.A. Ricupero et al., "Phosphatidylinositol 3-kinase-dependent stabilization of alpha(1)(I) collagen mRNA in human lung fibroblasts", AM J P-CELL, 281(1), 2001, pp. C99-C105

Abstract

We investigated the role of phosphatidylinositol 3-kinase (PI3K) in the expression of alpha (1)(I) collagen mRNA. We report that the basal level of alpha (1)(I) collagen mRNA was reduced when PI3K activity was inhibited by either LY-294002 or wortmannin. These PI3K inhibitors also blocked increasesof alpha (1)(I) collagen mRNA levels after the addition of transforming growth factor-beta. The effect of PI3K inhibition was abolished by the removal of the inhibitor or by the addition of cycloheximide. Inhibition of PI3K activity decreased the stability of the alpha (1)(I) collagen mRNA with no change in the rate of transcription of the alpha (1)(I) collagen gene as assessed by Northern blotting with actinomycin D-treated fibroblasts and nuclear run-on assays. Expression of a truncated alpha (1)(I) collagen minigenedriven by a cytomegalovirus promoter in murine fibroblasts was decreased by LY-294002 treatment. These data indicate that PI3K activation results in increased stabilization of alpha (1)(I) collagen mRNA. In vivo, the PI3K activity in fibroblasts may regulate basal levels of alpha (1)(I) collagen mRNA expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:43:23