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Titolo:
Identification of four novel potato (Solanum tuberosum) allergens belonging to the family of soybean trypsin inhibitors
Autore:
Seppala, U; Majamaa, H; Turjanmaa, K; Helin, J; Reunala, T; Kalkkinen, N; Palosuo, T;
Indirizzi:
Univ Helsinki, Inst Biotechnol, Prot Chem Lab, FIN-00014 Helsinki, FinlandUniv Helsinki Helsinki Finland FIN-00014 ab, FIN-00014 Helsinki, Finland Natl Publ Hlth Inst, Helsinki, Finland Natl Publ Hlth Inst Helsinki Finland Publ Hlth Inst, Helsinki, Finland Tampere Univ Hosp, Dept Dermatol, Tampere, Finland Tampere Univ Hosp Tampere Finland Hosp, Dept Dermatol, Tampere, Finland
Titolo Testata:
ALLERGY
fascicolo: 7, volume: 56, anno: 2001,
pagine: 619 - 626
SICI:
0105-4538(200107)56:7<619:IOFNP(>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CROSS-REACTIVITY; BIRCH POLLEN; RAW POTATO; ATOPIC-DERMATITIS; FOOD ALLERGENS; SKIN PRICK; CHILDREN; PROTEINS; VEGETABLES; BET-V-1;
Keywords:
allergen; children; food; IgE; potato; skin prick test; soybean trypsin inhibitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Seppala, U Univ Helsinki, Inst Biotechnol, Prot Chem Lab, POB 56, FIN-00014 Helsinki,Finland Univ Helsinki POB 56 Helsinki Finland FIN-00014 lsinki,Finland
Citazione:
U. Seppala et al., "Identification of four novel potato (Solanum tuberosum) allergens belonging to the family of soybean trypsin inhibitors", ALLERGY, 56(7), 2001, pp. 619-626

Abstract

Background: We have previously identified patatin (Sol t 1) of potato tubers as a major food allergen among atopic children. In addition to Sol t 1, concomitant ISE binding to other, then unidentified, potato proteins was observed. Methods: Purification and identification of the putative allergens were done by both standard and advanced methods of protein chemistry. The patient series comprised 39 children with positive skin prick test (SPT) to raw potato. Immunoblotting and ELISA were used to examine IgE-binding ability and skin prick testing to assess in vivo reactivity of the purified potato proteins. Results: Four IgE-binding potato proteins with molecular masses ranging from 16 to 20 kDa were purified and identified as cathepsin D-, cysteine-, and aspartic protease inhibitors belonging to the family of soybean trypsin inhibitors (Kunitz type). The proteins were designated Sol t 2, Sol t 3.0101, Sol t 3.0102, and Sol t 4. In ELISA, 51% of the sera of the 39 atopic children showed specific IgE to Sol t 2, 43% to Sol t 3.0101, 58% to Sol t 3.0102, and 67% to Sol t 4, respectively. All these four allergens were able to produce positive wheal-and-flare responses in SPT. Conclusions: In addition to Sol t 1, potato tubers contain several proteins belonging to the family of soybean trypsin inhibitors against which atopic children with positive SPT responses to raw potato have in vitro and in vivo reactive IgE antibodies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:01:00