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Titolo:
Kainate-induced epilepsy alters protein expression of AMPA receptor subunits GluR1, GluR2 and AMPA receptor binding protein in the rat hippocampus
Autore:
Sommer, C; Roth, SU; Kiessling, M;
Indirizzi:
Univ Heidelberg, Dept Neuropathol, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany
Titolo Testata:
ACTA NEUROPATHOLOGICA
fascicolo: 5, volume: 101, anno: 2001,
pagine: 460 - 468
SICI:
0001-6322(200105)101:5<460:KEAPEO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TEMPORAL-LOBE EPILEPSY; SELECTIVE GLUTAMATE RECEPTORS; KAINIC ACID; STATUS-EPILEPTICUS; GENE-EXPRESSION; MESSENGER-RNA; Q/R-SITE; NEUROLOGICAL DISORDERS; CA2+ PERMEABILITY; GLOBAL-ISCHEMIA;
Keywords:
alpha-amino-3-hydroxy-5-methyl-4-isoxazole; proprionic acid (AMPA) receptors; AMPA receptor binding protein (ABP); kainic acid; immunohistochemistry; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Sommer, C Univ Heidelberg, Dept Neuropathol, Neuenheimer Feld 220, D-69120Heidelberg, Germany Univ Heidelberg Neuenheimer Feld 220 Heidelberg Germany D-69120
Citazione:
C. Sommer et al., "Kainate-induced epilepsy alters protein expression of AMPA receptor subunits GluR1, GluR2 and AMPA receptor binding protein in the rat hippocampus", ACT NEUROP, 101(5), 2001, pp. 460-468

Abstract

Kainic acid induces seizures with consecutive degeneration of highly vulnerable hippocampal CA3 neurons in adult rats. An abnormal influx of calcium through newly synthesized alpha -amino-3-hydroxy-5-methyl-4-isoxazole proprionic acid (AMPA) receptors lacking the GluR2 subunit, which normally renders AMPA receptors calcium impermeable, is thought to play a pivotal role for postictal neuronal death (GluR2 hypothesis). Using a specific GluR2 antiserum, postictal hippocampal GluR2 protein expression was investigated and compared to GluR1 between 6 and 96 h after seizure induction. In addition, postictal protein expression of a recently cloned AMPA receptor binding protein (ABP), which anchors AMPA receptors in the plasma membrane was also analyzed, to address the question of whether its protein expression is associated with neuronal death or survival. At 6 h after seizure induction, GluR2 immunoreactivity (IR) in CA3 was more markedly reduced compared to GluR1, but at 24 h GluR2 IR reattained control levels. More importantly, GluR2 IR was also markedly, but transiently decreased between 6 and 48 h in hippocampal CA1 neurons, but no significant cell loss was observed. These findings modify the GluR2 hypothesis in so far as only a subset of, but not all, hippocampal CAI and CA3 pyramidal neurons may die due to reduced GluR2 levels with consecutive calcium overload through calcium-permeable AMPA receptors. ABP was induced postictally in presumed CA2 and a subpopulation of CA3 neurons and seems not to be involved in mechanisms of delayed neuronal death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:42:52