Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Crystallization and preliminary X-ray analysis of the C/EBP beta C-terminal region in complex with DNA
Autore:
Tahirov, TH; Inoue-Bungo, T; Sasaki, M; Fujikawa, A; Kimura, K; Sato, K; Adachi, S; Kamiya, N; Ogata, K;
Indirizzi:
Yokohama City Univ, Sch Med, KAST, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan Yokohama City Univ, Sch Med, Dept Biol Struct, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan Yokohama City Univ, Sch Med, Dept Urol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan Yokohama City Univ, Sch Med, Dept Biochem, Kanazawa Ku, Yokohama, Kanagawa2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 Kanagawa2360004, Japan RIKEN, Harima Inst, Biophys Chem Lab, Sayo, Hyogo 6795148, Japan RIKEN Sayo Hyogo Japan 6795148 ophys Chem Lab, Sayo, Hyogo 6795148, Japan RIKEN, Harima Inst, Biocrystallog Technol Div, Sayo, Hyogo 6795148, Japan RIKEN Sayo Hyogo Japan 6795148 og Technol Div, Sayo, Hyogo 6795148, Japan
Titolo Testata:
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
, volume: 57, anno: 2001,
parte:, 6
pagine: 854 - 856
SICI:
0907-4449(200106)57:<854:CAPXAO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTORS; V-MYB; BINDING; GENE; ACTIVATION; PROTEIN; SPECIFICITY; EXPRESSION; FAMILY; DOMAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Tahirov, TH Yokohama City Univ, Sch Med, KAST, Kanazawa Ku, 3-9 Fukuura, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ 3-9 Fukuura Yokohama Kanagawa Japan 2360004
Citazione:
T.H. Tahirov et al., "Crystallization and preliminary X-ray analysis of the C/EBP beta C-terminal region in complex with DNA", ACT CRYST D, 57, 2001, pp. 854-856

Abstract

The C-terminal fragment (residues 259-345) of human C/EBP beta, a basic region leucine zipper transcriptional regulatory factor which includes the minimal DNA-binding domain, was crystallized in complex with a 16 bp DNA fragment from the tom-1 promoter. The crystals were in the form of a parallelepiped belonging to space group C222(1), had unit-cell parameters a = 100.7 (2), b = 113.5 (1), c = 74.4 (1) Angstrom and diffracted to a resolution of 2.1 Angstrom. Moreover, truncation of nine residues from the C-terminus notconserved among C/EBP family members yielded isomorphous crystals that diffracted to a resolution of 1.8 Angstrom or better. Truncation of 14 residues from the N-terminus of the C-terminal fragment produced well shaped crystals in the form of hexagonal bipyramids, however; unfortunately, they were unstable and diffracted poorly.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 01:10:20