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Titolo:
Effects of vascular endothelial growth factor and E-selectin on angiogenesis in the murine metastatic RCT sarcoma
Autore:
Aoki, M; Kanamori, M; Yudoh, K; Ohmori, K; Yasuda, T; Kimura, T;
Indirizzi:
Toyama Med & Pharmaceut Univ, Dept Orthopaed Surg, Sugitani, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ Sugitani Toyama Japan 9300194 9300194, Japan
Titolo Testata:
TUMOR BIOLOGY
fascicolo: 4, volume: 22, anno: 2001,
pagine: 239 - 246
SICI:
1010-4283(200107/08)22:4<239:EOVEGF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILITY FACTOR; CELL-ADHESION; TUMOR-CELLS; IN-VITRO; EXPRESSION; CLONES; INVASIVENESS; RECEPTOR; LINES; MICE;
Keywords:
vascular endothelial growth factor; E-selectin; angiogenesis; metastasis; murine sarcoma; endothelial cell, murine lung;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Aoki, M Toyama Med & Pharmaceut Univ, Dept Orthopaed Surg, 2630 Sugitani, Sugitani, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ 2630 Sugitani Sugitani Toyama Japan 9300194
Citazione:
M. Aoki et al., "Effects of vascular endothelial growth factor and E-selectin on angiogenesis in the murine metastatic RCT sarcoma", TUMOR BIOL, 22(4), 2001, pp. 239-246

Abstract

The relationship between vascular endothelial growth factor (VEGF) and induction of angiogenesis in high-metastatic RCI(+) or low-metastatic RCT(-) clones of the poorly differentiated murine RCT sarcoma was investigated. Theassociation with E-selectin in VEGF-induced angiogenesis was also evaluated, RCT(+) cells produced significantly larger amounts of VEGF than RCT(-) cells. In a tube formation assay with murine lung microvascular endothelial (MLE) cells, conditioned medium from RCT(+) cells showed a significantly greater effect on tube formation than that from RCT(-) cells. Induction of tube formation was suppressed by anti-mouse VEGF monoclonal antibody, Furthermore, anti-mouse E-selectin monoclonal antibody suppressed the tube formation induced by recombinant mouse VEGF, In a flow-cytometric analysis, the expression of E-selectin on MLE cells was upregulated after pretreatment withconditioned medium from RCT(+) and RCT(-) cells. Conditioned medium from RCT(+) cells induced a higher expression of E-selectin compared to medium from RCT(-) cells. Anti-VEGF monoclonal antibody prevented the upregulation of E-selectin by the RCT cell-conditioned medium. These findings suggest that E-selectin plays an important role in the angiogenesis induced by VEGF, VEGF derived from tumor cells may enhance angiogenesis by upregulating the expression of E-selectin on vascular endothelial cells. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 21:08:40