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Titolo:
Cell proliferation in colorectal adenocarcinomas: Comparison between Ki-67immunostaining and bromodeoxyuridine uptake detected by immunohistochemistry and flow cytometry
Autore:
Le Pessot, F; Michel, P; Paresy, M; Lemoine, F; Hellot, MF; Paillot, B; Scotte, M; Peillon, C; Hemet, J;
Indirizzi:
Univ Rouen, Hosp Charles Nicolle, Dept Pathol, F-76031 Rouen, France Univ Rouen Rouen France F-76031 olle, Dept Pathol, F-76031 Rouen, France Univ Rouen, Hosp Charles Nicolle, ADEN EA3234, Digest Tract Res Grp, F-76031 Rouen, France Univ Rouen Rouen France F-76031 est Tract Res Grp, F-76031 Rouen, France Univ Rouen, Hosp Charles Nicolle, Dept Biostat, F-76031 Rouen, France UnivRouen Rouen France F-76031 lle, Dept Biostat, F-76031 Rouen, France Univ Rouen, Hosp Charles Nicolle, Dept Digest Surg, F-76031 Rouen, France Univ Rouen Rouen France F-76031 Dept Digest Surg, F-76031 Rouen, France Univ Rouen, Hosp Charles Nicolle, Dept Clin Surg, F-76031 Rouen, France Univ Rouen Rouen France F-76031 e, Dept Clin Surg, F-76031 Rouen, France
Titolo Testata:
PATHOLOGY RESEARCH AND PRACTICE
fascicolo: 6, volume: 197, anno: 2001,
pagine: 411 - 418
SICI:
0344-0338(2001)197:6<411:CPICAC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY KI-67; DOUBLING TIMES TPOT; BREAST-CANCER; COLONIC ADENOCARCINOMAS; PROGNOSTIC-SIGNIFICANCE; LABELING INDEXES; DNA-PLOIDY; S-PHASE; CARCINOMAS; TUMORS;
Keywords:
colorectal neoplasms; Ki-67 antigen; bromodeoxyuridine labeling index; flow cytometry; tumor proliferation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Le Pessot, F Univ Rouen, Hosp Charles Nicolle, Dept Pathol, Blvd Gambetta,F-76031 Rouen, France Univ Rouen Blvd Gambetta Rouen France F-76031 Rouen, France
Citazione:
F. Le Pessot et al., "Cell proliferation in colorectal adenocarcinomas: Comparison between Ki-67immunostaining and bromodeoxyuridine uptake detected by immunohistochemistry and flow cytometry", PATH RES PR, 197(6), 2001, pp. 411-418

Abstract

We compared three different means of assaying tumor proliferative activityin 30 human colorectal adenocarcinomas labeled in vivo with bromodeoxyuridine (Br-dUrd). The labeling indices (LI) of BrdUrd obtained both by flow cytometry (FCM) and immunohistochemistry (TH) were also compared with the labeling index of Ki-67. These methods were then related to tumor ploidy and pathological features. Flow cytometry was performed in accordance with Begg's method after intravenous infusion of BrdUrd four hours before surgery. Immunohistology was carried out on paraffin-embedded sections with monoclonal antibodies against BrdUrd and Ki-67. A positive correlation was found between BrdUrd LI obtained by both FMC and IH (p <0.0001), a finding that complies with the literature. However, we report on a correlation between Ki-67 LI and BrdUrd LIs in colorectal tumors (p=0.012). The results were valid for all tumors when they were subdivided into diploid and aneuploid groups. The labeling indices were significantly higher in the aneuploid turner group than in the diploid group (p=0.047). No relationship between proliferation parameters and tumor stage or grade was found. To our knowledge, this is the first report on a positive correlation between tumor proliferation indices in BrdUrd LIs and Ki-67 in colorectal carcinomas. This finding validates the value of Ki-67 immunostaining, which, however, should be confirmed in a larger series under the same technical conditions.

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Documento generato il 20/09/20 alle ore 07:05:17