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Titolo:
Arbuscular mycorrhiza in mini-mycorrhizotrons: first contact of Medicago truncatula roots with Glomus intraradices induces chalcone synthase
Autore:
Bonanomi, A; Oetiker, JH; Guggenheim, R; Boller, T; Wiemken, A; Vogeli-Lange, R;
Indirizzi:
Univ Basel, Inst Bot, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 nst Bot, CH-4056 Basel, Switzerland Univ Basel, REM Lab, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 REM Lab, CH-4056 Basel, Switzerland Syngenta AG, CH-4002 Basel, Switzerland Syngenta AG Basel Switzerland CH-4002 nta AG, CH-4002 Basel, Switzerland
Titolo Testata:
NEW PHYTOLOGIST
fascicolo: 3, volume: 150, anno: 2001,
pagine: 573 - 582
SICI:
0028-646X(200106)150:3<573:AMIMFC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIAL DISPLAY; DEFENSE RESPONSE; ALFALFA ROOTS; SATIVA L; EXPRESSION; GENES; INFECTION; FUNGI; ACCUMULATION; MICROCOMPUTER;
Keywords:
appressorium; early developmental stages of AM; environmental scanning electron microscopy (ESEM); in vitro; defence response; pathogens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Wiemken, A Univ Basel, Inst Bot, Hebelstr 1, CH-4056 Basel, Switzerland Univ Basel Hebelstr 1 Basel Switzerland CH-4056 l, Switzerland
Citazione:
A. Bonanomi et al., "Arbuscular mycorrhiza in mini-mycorrhizotrons: first contact of Medicago truncatula roots with Glomus intraradices induces chalcone synthase", NEW PHYTOL, 150(3), 2001, pp. 573-582

Abstract

The association of plants with arbuscular mycorrhizal (AM) fungi is widespread in nature, but little is known about molecular aspects of this symbiosis. Particularly during the early stages of the AM symbiosis, it is difficult to monitor growth of the two partners, to dissect gene expression patterns and to correlate them with plant, fungal or symbiosis development. A new system, the 'mini-mycorrhizotron', was established to cultivate seedlings of Medicago truncatula in mycorrhizal symbiosis with Glomus intraradices under gnotobiotic conditions. This system allows natural growth of the symbiotic partners and permits the continuous noninvasive observation of the development of plant and fungus under a microscope. The mini-mycorrhizotron was used to determine the stage of induction of a mycorrhiza-related gene detected by differential display-reverse transcription-PCR, namely a novel chalcone synthase (Mf-chs1). The gene is induced inroots at the stage of the first fungal contact. The mini-mycorrhizotron allowed identification and cloning of a symbiosis-related gene, and the correlation between its expression and the developmental stage of the symbiosis was established. This provides a useful tool formolecular and developmental studies of the early stages of AM symbioses. (C) New Phytologist (2001).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:41:52