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Titolo:
Characterization of ceramide-induced apoptotic death in cerebellar granulecells in culture
Autore:
Monti, B; Zanghellini, P; Contestabile, A;
Indirizzi:
Univ Bologna, Dept Biol, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 na, Dept Biol, I-40126 Bologna, Italy
Titolo Testata:
NEUROCHEMISTRY INTERNATIONAL
fascicolo: 1, volume: 39, anno: 2001,
pagine: 11 - 18
SICI:
0197-0186(200107)39:1<11:COCADI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; PROTEIN PHOSPHATASE; HIPPOCAMPAL-NEURONS; SIGNAL-TRANSDUCTION; NERVOUS-SYSTEM; PC12 CELLS; C-JUN; GENERATION; SURVIVAL; PATHWAY;
Keywords:
sphingomyelin pathway; apoptosis; DNA fragmentation; neurotoxicity; cerebellar granule cells; in vitro culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Contestabile, A Univ Bologna, Dept Biol, Via Selmi 3, I-40126 Bologna, Italy Univ Bologna Via Selmi 3 Bologna Italy I-40126 gna, Italy
Citazione:
B. Monti et al., "Characterization of ceramide-induced apoptotic death in cerebellar granulecells in culture", NEUROCHEM I, 39(1), 2001, pp. 11-18

Abstract

Sphingomyelin signalling system has been involved in several examples of cell death through apoptosis. We have characterised the effect of exposure to the cell permeable ceramide analogue, C-2-ceramide, on cultures of differentiated cerebellar granule cells. C-2-ceramide was toxic to granule cells in a dose- and time-dependent way at concentrations higher than 10 muM. Ceramide exposure was accompanied by characteristic alterations of cell morphology, namely swollen cell bodies and punctuate appearance and arcuate direction of processes. The final outcome of ceramide exposure was a form of cell death largely apoptotic in nature. Hoechst stain, followed by counts of nuclei with normal appearance and size or with condensed chromatin and reduced size, revealed a large increase of the proportion of shrunken nuclei in treated cultures. In situ visualisation of fragmented DNA through the TUNELtechnique, additionally marked cells undergoing apoptosis as a consequenceof ceramide treatment. accordingly, the DNA extracted from cultures exposed to C-2-ceramide and subjected to agarose gel electrophoresis showed the peculiar ladder of fragmented low molecular weight DNA. Treatments with inhibitors of two caspases or of nitric oxide synthase were unable to rescue neurons exposed to ceramide, thus suggesting a neurotoxic action not primarily dependent on activation of death proteases or on nitric oxide production. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 07:55:59