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Titolo:
Stimulated single fiber electromyography in the mouse: Techniques and normative data
Autore:
Gooch, CL; Mosier, DR;
Indirizzi:
Columbia Univ Coll Phys & Surg, New York, NY 10032 USA Columbia Univ Coll Phys & Surg New York NY USA 10032 w York, NY 10032 USA Baylor Coll Med, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ylor Coll Med, Houston, TX 77030 USA
Titolo Testata:
MUSCLE & NERVE
fascicolo: 7, volume: 24, anno: 2001,
pagine: 941 - 945
SICI:
0148-639X(200107)24:7<941:SSFEIT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYASTHENIA-GRAVIS; SAFETY FACTOR; JITTER; MUSCLE;
Keywords:
jitter; neuromuscular junction; single-fiber electromyography; vecuronium; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Gooch, CL Inst Neurol, EMG Lab, 710 W 168th St, New York, NY 10032 USA Inst Neurol 710 W 168th St New York NY USA 10032 k, NY 10032 USA
Citazione:
C.L. Gooch e D.R. Mosier, "Stimulated single fiber electromyography in the mouse: Techniques and normative data", MUSCLE NERV, 24(7), 2001, pp. 941-945

Abstract

As the number of new transgenic mouse models of human neuromuscular disease continues to increase, the development of sophisticated electrophysiologic techniques for assessing the peripheral nervous system in these models has become important. Neuromuscular junction (NMJ) dysfunction, in particular, is often not detectable by morphologic or other techniques. To enable sensitive testing of murine NMJ function, we developed and tested a method forstimulated single fiber electromyography (S-SFEMG) in the gastrocnemius muscles of anesthetized mice. Jitter was assessed by measuring the mean consecutive latency difference (MCD) of single fiber responses to sciatic nerve stimulation at 2 Hz. Mean MCD values in normothermic mice were in the rangeof 6-8 us for different strains, with no MCD values exceeding 25 mus. Reduced core temperature (to 29 degrees -30 degreesC) resulted in increased jitter, whereas intubation and mechanical ventilation of mice did not alter these values. Intraperitoneal and intravenous injection of vecuronium, however, resulted in progressively increased jitter followed by blocking in continuously monitored fibers. These observations validate the utility of S-SFEMG in mice as an index of NMJ function under a variety of physiologic conditions, and suggest that a high safety factor for neuromuscular transmission exists at mouse NMJs. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 08:13:25