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Titolo:
Heterologous expression of genes mediating enhanced fungal resistance in transgenic wheat
Autore:
Oldach, KH; Becker, D; Lorz, H;
Indirizzi:
Univ Hamburg, AMPII, Inst Appl Mol Plant Biol, D-22609 Hamburg, Germany Univ Hamburg Hamburg Germany D-22609 lant Biol, D-22609 Hamburg, Germany
Titolo Testata:
MOLECULAR PLANT-MICROBE INTERACTIONS
fascicolo: 7, volume: 14, anno: 2001,
pagine: 832 - 838
SICI:
0894-0282(200107)14:7<832:HEOGME>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOME-INACTIVATING PROTEINS; PATHOGENESIS-RELATED PROTEINS; ASPERGILLUS-GIGANTEUS; ANTIFUNGAL-PROTEIN; CHITINASE GENE; PLANTS; TOBACCO; DEFENSE; BETA-1,3-GLUCANASE; BOMBARDMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Lorz, H Univ Hamburg, AMPII, Inst Appl Mol Plant Biol, Ohnhorststr 18, D-22609 Hamburg, Germany Univ Hamburg Ohnhorststr 18 Hamburg Germany D-22609 burg, Germany
Citazione:
K.H. Oldach et al., "Heterologous expression of genes mediating enhanced fungal resistance in transgenic wheat", MOL PL MICR, 14(7), 2001, pp. 832-838

Abstract

Three cDNAs encoding the antifungal protein Ag-AFP from the fungus Aspergillus giganteus, a barley class II chitinase and a barley type I RIP, all regulated by the constitutive Ubiquitin1 promoter from maize, were expressed in transgenic wheat. In 17 wheat lines, stable integration and inheritance of one of the three transgenes has been demonstrated over four generations. The formation of powdery mildew (Erysiphe graminis f. sp, tritici) or leafrust (Puccinia recondita f. sp, tritici) colonies was significantly reduced on leaves from afp or chitinase II- but not from rip I-expressing wheat lines compared with nontransgenic controls. The increased resistance of afp and chitinase II lines was dependent on the dose of fungal spores used for inoculation. Heterologous expression of the fungal afp gene and the barley chitinase II gene in wheat demonstrated that colony formation and, thereby,spreading of two important biotrophic fungal diseases is inhibited approximately 40 to 50% at an inoculum density of 80 to 100 spores per cm(2).

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Documento generato il 02/04/20 alle ore 18:59:57