Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Production of secretory leukocyte protease inhibitor by human amniotic membranes and regulation of its concentration in amniotic fluid
Autore:
Zhang, Q; Shimoya, K; Moriyama, A; Yamanaka, K; Nakajima, A; Nobunaga, T; Koyama, M; Azuma, C; Murata, Y;
Indirizzi:
Osaka Univ, Fac Med, Dept Obstet & Gynecol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Gynecol, Suita, Osaka 5650871, Japan Saiseikai Nakatsu Hosp, Dept Obstet & Gynecol, Osaka 5438502, Japan Saiseikai Nakatsu Hosp Osaka Japan 5438502 Gynecol, Osaka 5438502, Japan
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 6, volume: 7, anno: 2001,
pagine: 573 - 579
SICI:
1360-9947(200106)7:6<573:POSLPI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; LEUKOPROTEASE INHIBITOR; EPITHELIAL-CELLS; BACTERIAL LIPOPOLYSACCHARIDE; SLPI CONCENTRATIONS; POTENT INHIBITOR; GENE-EXPRESSION; CERVICAL-MUCUS; ELASTASE; INTERLEUKIN-1-BETA;
Keywords:
amnion; amniotic fluid; amniotic membrane; secretory leukocyte protease inhibitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Shimoya, K Osaka Univ, Fac Med, Dept Obstet & Gynecol, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 50871, Japan
Citazione:
Q. Zhang et al., "Production of secretory leukocyte protease inhibitor by human amniotic membranes and regulation of its concentration in amniotic fluid", MOL HUM REP, 7(6), 2001, pp. 573-579

Abstract

Secretory leukocyte protease inhibitor (SLPI) is a potent inhibitor of human leukocyte elastase, SLPI is a protein found in various human fluids, including parotid secretions, cervical mucus, seminal plasma and ascites, Western blot analysis revealed that SLPI protein is detected as a 12 kDa band in both the amniotic fluid and the amniotic membrane. The amniotic fluid concentrations of SLPI were assayed by enzyme-linked immunosorbent assay. SLPIconcentrations in the amniotic fluid of women in the third trimester were higher than those in the second trimester. Immunohistochemistry using an anti-SLPI polyclonal antibody revealed positive staining in epithelial cells in amniotic membranes. Reverse transcription-polymerase chain reaction demonstrated that SLPI transcripts could be detected in the amniotic membranes. To determine the mechanism of SLPI production by amniotic cells, purified amniotic cells were stimulated with various cytokines, Amniotic cells produced SLPI in a dose-dependent manner when stimulated with interleukin (IL)-1alpha, IL-1 beta, and tumour necrosis factor-alpha. The present findings show that SLPI is produced by the amniotic membranes in response to cytokineconcentrations. The SLPI in the amniotic fluid may contribute to immunodefence mechanisms during pregnancy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 04:43:18