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Titolo:
Leptin and leptin receptor mRNA are widely expressed in tumors of adipocytic differentiation
Autore:
Oliveira, AM; Nascimento, AG; Lloyd, RV;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 thol, Rochester, MN 55905 USA
Titolo Testata:
MODERN PATHOLOGY
fascicolo: 6, volume: 14, anno: 2001,
pagine: 549 - 555
SICI:
0893-3952(200106)14:6<549:LALRMA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
BROWN ADIPOSE-TISSUE; OBESE GENE-EXPRESSION; BODY-WEIGHT; MESSENGER-RNA; DIABETIC MICE; OB/OB MICE; RAT BROWN; OB RNA; WHITE; CELLS;
Keywords:
hibernoma; leptin; leptin receptor (OB-Rb); lipoma; liposarcoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Lloyd, RV Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, 200 1st St SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW Rochester MN USA 55905 05 USA
Citazione:
A.M. Oliveira et al., "Leptin and leptin receptor mRNA are widely expressed in tumors of adipocytic differentiation", MOD PATHOL, 14(6), 2001, pp. 549-555

Abstract

Adipose tissue is the principal source of leptin, a cytokine-like peptide with many biologic functions. Leptin binds to the leptin receptor, present in the hypothalamus and in many other tissues, and modulates energy balanceand maintenance of body weight. The expression of leptin and leptin receptor in tumors of adipocytic differentiation has not been previously examined. Because normal adipose tissue is the principal source of leptin and expresses leptin receptor, we hypothesized that tumors of adipose tissue differentiation may also express leptin and/or the long functional form of the leptin receptor (OB-Rb). Leptin and OB-Rb were analyzed by immunohistochemistry, in situ. hyhridization, RT-PCR, and western blotting in 21 lipomas, 2 hibernomas, and 16 liposarcomas. Immunostaining and in situ hybridization showed leptin and OB-Rb mRNA expression in all cases of lipomas, hibernomas, and liposarcomas, including dedifferentiated and pleomorphic liposarcomas. RT-PCR analysis showedleptin and OB-Rb mRNA in both lipomas (ut = 5) and liposarcomas (n = 5). Western blotting identified the 16 kDa leptin protein in a lipoma and a liposarcoma No important difference in the expression of leptin and OB-Rb mRNA was found between lipomas and liposarcomas, although the level of leptin protein was higher in a lipoma than a liposarcoma by western blotting. These results show for the first time that leptin and OB-Rb mRNA are expressed in lipomas, hibernomas, and liposarcomas. The presence of leptin and its receptor may provide new insights into the pathobiology of these tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 10:23:51