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Titolo:
Potentiation of nerve growth factor-induced elongation of neurites by gelsemiol and 9-hydroxysemperoside aglucone in PC12D cells
Autore:
Li, YS; Matsunaga, K; Kato, R; Ohizumi, Y;
Indirizzi:
Tohoku Univ, Grad Sch Pharmaceut Sci, Dept Pharmaceut Mol Biol, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Ku, Sendai, Miyagi 9808578, Japan
Titolo Testata:
JOURNAL OF PHARMACY AND PHARMACOLOGY
fascicolo: 6, volume: 53, anno: 2001,
pagine: 915 - 919
SICI:
0022-3573(200106)53:6<915:PONGFE>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ASTROCYTOMA-CELLS; ALZHEIMERS-DISEASE; OUTGROWTH; ATPASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Ohizumi, Y Tohoku Univ, Grad Sch Pharmaceut Sci, Dept Pharmaceut Mol Biol,Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Miyagi 9808578, Japan
Citazione:
Y.S. Li et al., "Potentiation of nerve growth factor-induced elongation of neurites by gelsemiol and 9-hydroxysemperoside aglucone in PC12D cells", J PHARM PHA, 53(6), 2001, pp. 915-919

Abstract

In PC12D cells, nerve growth factor (NGF) increased the proportion of neurite-bearing cells and made neurites longer. A methanol extract of Verbena littoralis H. B. K. collected in Paraguay only slightly potentiated the proportion of PC12D cells with neurites but markedly increased the length of neurites in the presence of NGF (2 ng ml(-1)). The methanol extract was partitioned between ethyl acetate and water followed by further extraction of water fraction with n-butanol. The potentiating activity of NGF-action was observed in the ethyl acetate and n-butanol fractions. The n-butanol fractionwas separated by silica gel chromatography, monitoring the NGF-potentiating activity to give gelsemiol and 9-hydroxysemperoside aglucone (9-OHSA). Neither compound (30-300 muM) exhibited neurite-inducing activity alone. Gelsemiol (100-300 muM) markedly enhanced an increase in the proportion of neurite-bearing cells and an extension of the neurite length in the presence ofNGF (2 ng mL(-1)). Interestingly, in the presence of NGF (2 ng ml(-1)), 9-OHSA (100-300 muM) enhanced the elongation of neurites without affecting the increase in the proportion of cells with neurites. These results suggested that gelsemiol and 9-OHSA were major active components of V. littoralis in the NGF-potentiating action. It was possible that the mechanism of neurite elongation by NGF was different from that of the increase in the proportion of neurite-bearing cells, and that 9-OHSA selectively affected the neurite elongation mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:41:08