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Titolo:
Analysis of steroid-induced genes in the rat preoptic area-anterior hypothalamus using a differential-display reverse transcriptase-polymerase chain reaction
Autore:
Park, S; Seong, JY; Son, GH; Kang, SS; Lee, S; Kim, SR; Kim, K;
Indirizzi:
Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 Sci, Seoul 151742, South Korea Chonnam Natl Univ, Hormone Res Ctr, Kwangju, South Korea Chonnam Natl Univ Kwangju South Korea one Res Ctr, Kwangju, South Korea Gyeongsang Natl Univ, Coll Med, Dept Anat, Chinju, South Korea Gyeongsang Natl Univ Chinju South Korea Dept Anat, Chinju, South Korea Ehwa Womans Univ, Coll Med, Dept Med, Seoul, South Korea Ehwa Womans UnivSeoul South Korea ll Med, Dept Med, Seoul, South Korea
Titolo Testata:
JOURNAL OF NEUROENDOCRINOLOGY
fascicolo: 6, volume: 13, anno: 2001,
pagine: 531 - 539
SICI:
0953-8194(200106)13:6<531:AOSGIT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR MESSENGER-RNA; MEDIOBASAL HYPOTHALAMUS; RIBONUCLEIC-ACID; SEXUAL-BEHAVIOR; FEMALE RATS; ESTROGEN; EXPRESSION; HORMONE; PROGESTERONE; IDENTIFICATION;
Keywords:
steroids; genes; DD-PCR; POA-AH; puberty; oestrous cycle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, K Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea Seoul NatlUniv Seoul South Korea 151742 eoul 151742, South Korea
Citazione:
S. Park et al., "Analysis of steroid-induced genes in the rat preoptic area-anterior hypothalamus using a differential-display reverse transcriptase-polymerase chain reaction", J NEUROENDO, 13(6), 2001, pp. 531-539

Abstract

Steroid hormones modulate a variety of physiological functions in the hypothalamus. We attempted to identify steroid-regulated genes in the rat preoptic area-anterior hypothalamus by comparing differentially expressed mRNAs,Adult female rats were ovariectomized and, 1 week later, a silastic capsule containing 17 beta -oestradiol (180 mug/ml) was subcutaneously implanted. After 2 days, a single injection of progesterone (1 mg) was administered at 10.00 h and rats were killed at 17.00 h on the same day, Differential-display polymerase chain reaction followed by Northern blot analysis showed that 10 clones were differentially regulated. Using homology search in Genbank, three genes were identified as sodium, potassium-ATPase beta1, protein kinase C-binding Nell-homologue protein and evectin-1, Further characterization of 10 clones showed that the expression patterns were tissue-specific and differentially regulated during puberty. Among these, mRNAs for protein kinase C-binding Nell-homologue protein, evectin-1 and human CGI-118 protein-like gene were induced after vagina opening, and differentially expressedduring the oestrous cycle. Taken together, several steroid-regulated genesidentified in the present study may play an important role in regulating hypothalamic functions, including puberty and the oestrous cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 05:12:45