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Titolo:
Preparation of no-carrier-added [I-124]A(14)-iodoinsulin as a radiotracer for positron emission tomography
Autore:
Glaser, M; Brown, DJ; Law, MP; Iozzo, P; Waters, SL; Poole, K; Knickmeier, M; Camici, PG; Pike, VW;
Indirizzi:
Hammersmith Hosp, Imaging Res Solut Ltd, London W12 0NN, England Hammersmith Hosp London England W12 0NN lut Ltd, London W12 0NN, England Hammersmith Hosp, MRC, Ctr Clin Sci, London W12 0NN, England Hammersmith Hosp London England W12 0NN lin Sci, London W12 0NN, England CNR, Inst Clin Physiol, I-56126 Pisa, Italy CNR Pisa Italy I-56126CNR, Inst Clin Physiol, I-56126 Pisa, Italy Univ Munster, Dept Nucl Med, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 Nucl Med, D-48149 Munster, Germany
Titolo Testata:
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
fascicolo: 6, volume: 44, anno: 2001,
pagine: 465 - 480
SICI:
0362-4803(200105)44:6<465:PON[AA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; INSULIN; I-124; RATS;
Keywords:
iodine-124; iodine-125; human insulin; iodoinsulin; specific radioactivity; PET;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Pike, VW NIMH, Mol Imaging Branch, Bldg 1,Room B3-20,1 Ctr Dr,MSC 0135, Bethesda, MD 20892 USA NIMH Bldg 1,Room B3-20,1 Ctr Dr,MSC 0135 Bethesda MD USA 20892 SA
Citazione:
M. Glaser et al., "Preparation of no-carrier-added [I-124]A(14)-iodoinsulin as a radiotracer for positron emission tomography", J LABEL C R, 44(6), 2001, pp. 465-480

Abstract

A(14)-Iodoinsulin is a close biochemical and pharmacological mimic of insulin. Human insulin was labelled in its A chain tyrosine-14 residue by direct iodination with the positron-emitter iodine-124 (beta (+) = 25.6%; t(1/2)= 4.15 days) to provide a radiotracer for imaging with positron emission tomography (PET). Several reagents were compared for conversion of cyclotron-produced [I-124]iodide into a reactive species for the labelling reaction. Radiochemical yields from the use of Iodo-Gen (R), Chloramine-T, N-bromosuccinimide or lactoperoxidase-hydrogen peroxide were similar [35% (n = 1), 33 +/- 9% (n = 10), 32 +/- 11% (n = 25) or 33% (n = 2), respectively]. [I-124]A(14)-Iodoinsulin was separated from unreacted insulin and radioactive by-products by tandem reverse phase HPLC and rapidly formulated for intravenous injection by adsorption on a Sep-Pak (t)C18-Plus (R) cartridge, followedby elution with 10 mM hydrochloric acid-ethanol (1 : 1 v/v, 1 mi). This radiotracer can now be obtained in useful radioactivities at high effective specific radioactivity and is now being applied to PET studies of its biodistribution in living subjects. Copyright (C) 2001 John Wiley & Sons, Ltd.

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Documento generato il 13/07/20 alle ore 09:57:29