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Titolo:
Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in C-13/N-15 labeled RNA
Autore:
Hu, WD; Gosser, YQ; Xu, WJ; Patel, DJ;
Indirizzi:
Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr New York NY USA 10021 New York, NY 10021 USA
Titolo Testata:
JOURNAL OF BIOMOLECULAR NMR
fascicolo: 2, volume: 20, anno: 2001,
pagine: 167 - 172
SICI:
0925-2738(2001)20:2<167:N2A3MB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HETERONUCLEAR MULTIDIMENSIONAL NMR; TRIPLE-RESONANCE NMR; NUCLEIC-ACIDS; SIMULTANEOUS ACQUISITION; SINGLE 3D; OLIGONUCLEOTIDES; PROTEINS; C-13; SENSITIVITY; COHERENCE;
Keywords:
base-sugar proton/carbon correlation; bi-directional HCNCH; multiple-quantum coherence; RNA oligomers;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Hu, WD Mem Sloan Kettering Canc Ctr, 1275 York Ave, New York, NY 10021 USAMem Sloan Kettering Canc Ctr 1275 York Ave New York NY USA 10021 SA
Citazione:
W.D. Hu et al., "Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in C-13/N-15 labeled RNA", J BIOM NMR, 20(2), 2001, pp. 167-172

Abstract

Multiple-quantum 2D and 3D bi-directional HCNCH experiments are presented for the correlation of base and ribose protons/carbons in C-13/N-15 labeledHIV-1 TAR RNA. In both 2D and 3D experiments, the magnetization of H1' is transferred to H6/H8 and H1' through H1'-C1'-N1/9-C6/8-H6/8 and H1'-C1'-N1/9-C1'-H1' pathways, and the magnetization of H6/8 is transferred to H1' andH6/8 through H6/8-C6/8-N1/9-C1'-H1' and H6/8-C6/8-N1/9-C6/8-H6/8 pathways. Chemical shifts of four different nuclei (H1', C1', C6/8 and H6/8) are sampled in the 2D experiment. The correlation of base and ribose protons/carbons is established by the rectangular arrangement of crossover and out-and-back peaks in the proton/carbon correlated spectrum. The rectangular connections can be further resolved using the nitrogen dimension in a H-1/C-13/N-15 3D experiment. Furthermore, by taking advantage of the well separated chemical shifts of N1 (pyrimidine) and N9 (purine), the 2D spectrum can be simplified into two sub-spectra based on their base type. Both experiments were tested on a C-13/N-15 labeled 27-mer HIV-1 TAR RNA containing a UUCG hairpin loop.

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Documento generato il 05/07/20 alle ore 09:20:34