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Titolo:
Interdependence of cdk2 activation and Interleukin-2R alpha accumulation in T cells
Autore:
Mohapatra, S; Pledger, WJ;
Indirizzi:
Univ S Florida, Coll Med, H Lee Moffitt Canc Ctr & Res Inst, Mol Oncol Program, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 Mol Oncol Program, Tampa, FL 33612 USA Univ S Florida, Coll Med, Dept Oncol, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 ll Med, Dept Oncol, Tampa, FL 33612 USA Univ S Florida, Coll Med, Dept Biochem & Mol Biol, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 Biochem & Mol Biol, Tampa, FL 33612 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 24, volume: 276, anno: 2001,
pagine: 21984 - 21989
SICI:
0021-9258(20010615)276:24<21984:IOCAAI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIN-DEPENDENT KINASES; IL-2 RECEPTOR; GAMMA-CHAIN; TRANSCRIPTION FACTOR; MESSENGER-RNA; GROWTH-FACTOR; EXPRESSION; LYMPHOCYTES; P27(KIP1); INHIBITOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Pledger, WJ Univ S Florida, Coll Med, H Lee Moffitt Canc Ctr & Res Inst, Mol Oncol Program, 12902 Magnolia Dr, Tampa, FL 33612 USA Univ S Florida 12902 Magnolia Dr Tampa FL USA 33612 33612 USA
Citazione:
S. Mohapatra e W.J. Pledger, "Interdependence of cdk2 activation and Interleukin-2R alpha accumulation in T cells", J BIOL CHEM, 276(24), 2001, pp. 21984-21989

Abstract

We have shown previously that serum promotes T cell proliferation by acting with T cell receptor (TCR) agonists to efficiently down-regulate p27(Kip1) and activate cdk2-containing complexes. In the studies described here, the effect of serum on the expression of the alpha subunit of the interleukin-2 receptor (IL-2R alpha) was examined. We found that serum was required for maximal and sustained IL-2R alpha protein expression and consequent IL-2 signaling in TCR-activated splenocytes. Serum had no effect on IL-2R alpha mRNA levels and thus modulates IL-2R alpha expression post-transcriptionally. Unlike wild-type splenocytes, splenocytes exhibiting serum-independent cdk2 activation due to loss of p27(Kip1) efficiently expressed IL-2R alpha in serum-deficient medium. Conversely, serum did not promote IL-2R alpha accumulation in conditions in which cdk2 activity was blocked. These findings demonstrate that cdk2 activation is necessary and sufficient for IL-2R alpha accumulation in TCR-stimulated splenocytes. On the other hand, IL-2 signaling was required (at least in part) for cdk2 activation in these cells. Thus, cdk2 activation, IL-2R alpha expression, and IL-2 signaling are interdependent events, and we suggest that this feed-forward regulatory loop playsa key role in T cell mitogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 03:26:51