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Titolo:
The apolipoprotein E-dependent low density lipoprotein cholesteryl ester selective uptake pathway in murine adrenocortical cells involves chondroitinsulfate proteoglycans and an alpha(2)-macroglobulin receptor
Autore:
Swarnakar, S; Beers, J; Strickland, DK; Azhar, S; Williams, DL;
Indirizzi:
SUNY Stony Brook, Univ Med Ctr, Dept Pharmacol Sci, Stony Brook, NY 11794 USA SUNY Stony Brook Stony Brook NY USA 11794 Sci, Stony Brook, NY 11794 USA Amer Red Cross, Holland Lab, Rockville, MD 20855 USA Amer Red Cross Rockville MD USA 20855 olland Lab, Rockville, MD 20855 USA Palo Alto Hlth Care Syst, Vet Affairs, Ctr Geriatr Res Educ & Clin, Palo Alto, CA 94304 USA Palo Alto Hlth Care Syst Palo Alto CA USA 94304 , Palo Alto, CA 94304 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 24, volume: 276, anno: 2001,
pagine: 21121 - 21128
SICI:
0021-9258(20010615)276:24<21121:TAELDL>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
E MESSENGER-RNA; SECRETION-CAPTURE ROLE; I SR-BI; SCAVENGER RECEPTOR; PERIPHERAL-TISSUES; LUTEINIZED OVARY; SURFACE PROTEOGLYCANS; REMNANT LIPOPROTEINS; RICH LIPOPROTEINS; NONHUMAN-PRIMATES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Williams, DL SUNY Stony Brook, Univ Med Ctr, Dept Pharmacol Sci, Stony Brook, NY 11794 USA SUNY Stony Brook Stony Brook NY USA 11794 rook, NY 11794 USA
Citazione:
S. Swarnakar et al., "The apolipoprotein E-dependent low density lipoprotein cholesteryl ester selective uptake pathway in murine adrenocortical cells involves chondroitinsulfate proteoglycans and an alpha(2)-macroglobulin receptor", J BIOL CHEM, 276(24), 2001, pp. 21121-21128

Abstract

Cells acquire lipoprotein cholesterol by receptor-mediated endocytosis andselective uptake pathways. In the latter case, lipoprotein cholesteryl ester (CE) is transferred to the plasma membrane without endocytosis and degradation of the lipoprotein particle. Previous studies with Y1/E/tet/2/3 murine adrenocortical cells that were engineered to express apolipoprotein (apo) E demonstrated that apoE expression enhances low density lipoprotein (LDL) CE uptake by both selective and: endocytic pathways. The present experiments test the hypothesis that apoE-dependent LDL CE selective up take is mediated by scavenger receptor, class B, type I (SR-BI), Surprisingly, SR-BI expression was not detected in the Y1/E/tet/2/3 clone of Y1 adrenocortical cells, indicating the presence of a distinct apoE-dependent pathway for LDL CE selective uptake. ApoE-dependent LDL CE selective uptake in Y1/E/tet/2/3cells was inhibited by receptor-associated protein and by activated alpha (2)-macroglobulin (alpha M-2), suggesting the participation of the LDL receptor-related protein/alpha M-2 receptor. Reagents that inhibited proteoglycan synthesis or removed cell surface chondroitin sulfate proteoglycan completely blocked apoE-dependent LDL CE selective uptake. None of these reagents inhibited SR-BI-mediated LDL CE selective uptake in the Y1-BS1 clone of Y1 cells in which LDL CE selective uptake is mediated by GR-BI. We conclude that LDL CE selective uptake in adrenocortical cells occurs via SR-BI-independent and SR-BI-dependent pathways. The SR-BI-independent pathway is an apoE-dependent process that involves both chondroitin sulfate proteoglycans and an alpha M-2 receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 20:41:00