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Titolo:
Eosinophil transmigration across VCAM-1-expressing endothelial cells is upregulated by antigen-stimulated mononuclear cells
Autore:
Nagata, M; Yamamoto, H; Tabe, K; Sakamoto, Y;
Indirizzi:
Saitama Med Sch, Dept Internal Med 2, Div Pulm, Moroyama, Saitama 3500495,Japan Saitama Med Sch Moroyama Saitama Japan 3500495 ama, Saitama 3500495,Japan
Titolo Testata:
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
, volume: 125, anno: 2001, supplemento:, 1
pagine: 7 - 11
SICI:
1018-2438(2001)125:<7:ETAVEC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LATE ACTIVATION ANTIGEN-4; DIFFERENTIAL REGULATION; LYMPHOCYTE FUNCTION; ADHESION MOLECULES; ALLERGEN CHALLENGE; RANTES; RECRUITMENT; EXPRESSION; CHEMOKINES; VCAM-1;
Keywords:
eosinophils; endothelial cells; vascular cell adhesion molecule 1; transmigration; mononuclear cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Nagata, M Saitama Med Sch, Dept Internal Med 2, Div Pulm, 38 Morohongou, Moroyama, Saitama 3500495, Japan Saitama Med Sch 38 Morohongou Moroyama Saitama Japan 3500495 pan
Citazione:
M. Nagata et al., "Eosinophil transmigration across VCAM-1-expressing endothelial cells is upregulated by antigen-stimulated mononuclear cells", INT A AL IM, 125, 2001, pp. 7-11

Abstract

Background: Adhesion to and transmigration across endothelial cells expressing vascular cell adhesion molecule 1 (VCAM-1) may be key steps in the development of selective eosinophil accumulation at the allergic inflammation sites. There is evidence that cytokines/chemokines produced by CD4+ T cellsplay a prominent role in these processes. Objective: The objective of thisstudy was to evaluate whether eosinophil migration across human pulmonary microvascular endothelial cells (HPMEC) expressing VCAM-1 is modulated by the supernatants of antigen-stimulated mononuclear cells obtained from atopic asthmatics. Methods: Peripheral blood mononuclear cells (PBMC) were isolated from Dermatophagoides farinae (Df)-sensitive asthmatic subjects and cultured for 96 h at 37 degreesC in the presence or absence of 1 mug/ml Df antigen. Eosinophils were isolated from blood of healthy subjects and placed on the HPMEC monolayers cultured on a transwell filter (3-mum pore size) stimulated with IL-4 plus TNF-alpha (both at 100 pM, 24 h) The supernatants ofPBMC were then applied to the lower compartment and the transmigration of eosinophils was examined. Results: The supernatants of PBMC stimulated withDf significantly enhanced the eosinophil transmigration across VCAM-1-expressing HPMEC (% migration: 7.6 +/- 0.6 by the supernatants of PBMC culturedwithout Df vs. 12.3 +/- 1.2 by the PBMC cultured with Df, p < 0.01, n = 8). The enhanced migration, but not spontaneous migration, was blocked by theanti-<alpha>(4) integrin antibody. Moreover, the enhanced transmigration was blocked by anti-CCR3 antibody. Conclusion: The antigen-stimulated PBMC from atopic asthmatics produce an activity to induce eosinophil migration across VCAM-1-expressing endothelial cells. This activity appears to involve CCR3 as an essential molecule. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:52:21