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Titolo:
Transforming growth factor beta may act as an autocrine-survival-promotingfactor for transformed trophoblasts
Autore:
Ho, CK; Au, LC; Peng, HJ; Wang, SY;
Indirizzi:
Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan Vet Gen Hosp Taipei Taiwan en Hosp, Dept Med Res & Educ, Taipei, Taiwan Natl Yang Ming Univ, Inst Tradit Med, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 nst Tradit Med, Taipei 112, Taiwan
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 4, volume: 37, anno: 2001,
pagine: 245 - 250
SICI:
1071-2690(200104)37:4<245:TGFBMA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
TGF-BETA; CHORIOCARCINOMA CELLS; PROTEOGLYCAN DECORIN; ADENYLYL-CYCLASE; GENE-EXPRESSION; HUMAN-PLACENTA; CANCER CELLS; DIFFERENTIATION; RECEPTORS; PROTEIN;
Keywords:
TGF-beta; trophoblasts; decorin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Ho, CK Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan Vet Gen Hosp Taipei Taiwan , Dept Med Res & Educ, Taipei, Taiwan
Citazione:
C.K. Ho et al., "Transforming growth factor beta may act as an autocrine-survival-promotingfactor for transformed trophoblasts", IN VITRO-AN, 37(4), 2001, pp. 245-250

Abstract

Using five trophoblast cell lines of different differentiation status, we have shown that trophoblasts could constitutively release the transforming growth factor beta-1 (TGF beta1), but not TGF beta2. Treatment of the humantumorigenic, TL, and BeWo cells with a differentiating agent and a potent protein kinase C activator-the tumor-promoting agent-or the JEG-3 cells with cholera toxin-a potent cyclic adenosine 3':5'monophosphate (cAMP) inducer-or its analogue 8-bromo-cAMP, potentiates TGF beta production, but the twosignaling pathways appear to be mutually exclusive. Surprisingly, the JAR cell line failed to respond to either type of TGF beta activator. Based on reverse transcriptase (RT)-polymerase chain reaction (PCR), it was found that only the JAR cell line expressed messenger ribonucleic acid for decorin,a natural inhibitor of TGF beta, and none of the cell lines had detectableprotein expression as determined by immunocytochemical studies. The cell number in cultures with decorin was invariably lesser than in those without decorin under serum-free conditions for all the cell. lines tested. These results suggest that TGF beta may act as an autocrine-survival factor for transformed trophoblasts by allowing the cells to survive longer under a microenvironment which is not favorable for growth. Lastly, our results indicate that decorin, acting in a paracrine manner, may also play an important negative regulatory role in the development of transformed trophoblasts by sequestering TGF beta, thereby preventing its action.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 17:20:24