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Titolo:
Simulated microgravity impairs respiratory burst activity in human promyelocytic cells
Autore:
Hughes, JH; Long, JP;
Indirizzi:
Childrens Res Inst, Columbus, OH 43205 USA Childrens Res Inst Columbus OHUSA 43205 Res Inst, Columbus, OH 43205 USA Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 & Med Genet, Columbus, OH 43210 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 4, volume: 37, anno: 2001,
pagine: 209 - 215
SICI:
1071-2690(200104)37:4<209:SMIRBA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROTATING-WALL VESSEL; PROTEIN-KINASE-C; T-LYMPHOCYTES; IMMUNE-SYSTEM; 3-DIMENSIONAL CULTURE; DIHYDRORHODAMINE 123; NEUTROPHIL FUNCTION; SPACE-FLIGHT; ACTIVATION; EXPRESSION;
Keywords:
reactive oxygen intermediates; innate immunity; HL-60 cells; high-aspect ratio vessels; dihydrorhodamine 123;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Hughes, JH Childrens Res Inst, 308 Wexner Inst,700 Childrens Dr, Columbus,OH 43205 USA Childrens Res Inst 308 Wexner Inst,700 Childrens Dr Columbus OH USA 43205
Citazione:
J.H. Hughes e J.P. Long, "Simulated microgravity impairs respiratory burst activity in human promyelocytic cells", IN VITRO-AN, 37(4), 2001, pp. 209-215

Abstract

The concept of microgravity (free-fall) influencing cellular functions in nonadherent cells has not been a part of mainstream scientific thought. Utilizing rotating wall vessels (RWVs) to generate simulated microgravity conditions, we found that respiratory burst activity was significantly altered in nonadherent promyelocytic (HL-60) cells. Specifically, HL-60 cells in simulated microgravity for 6, 19, 42, 47, and 49 d had 3.8-fold fewer cells that were able to participate in respiratory burst activity than cells from 1 x g cultures (P = 0.0011, N = 5). The quantity of respiratory burst products from the cells in simulated microgravity was also significantly reduced. The fold increase over controls in mean fluorescence intensities for oxidative products from cells in microgravity was 1.1 +/- 0.1 versus 1.8 +/- 0.3 for cells at 1 X g (P = 0.013, N = 4). Furthermore, the kinetic response for phorbol ester-stimulated burst activity was affected by simulated microgravity. These results demonstrate that simulated microgravity alters an innate cellular function (burst activity). If respiratory burst activity is impaired by true microgravity, then recovery from infections during spaceflight could be delayed. Finally, RWVs provide an excellent model for investigating the mechanisms associated with microgravity-induced changes in nonadherent cells.

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Documento generato il 30/11/20 alle ore 12:29:49