Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Long-term engraftment of nonobese diabetic/severe combined immunodeficientmice with human CD34(+) cells transduced by a self-inactivating human immunodeficiency virus type 1 vector
Autore:
Gatlin, J; Padgett, A; Melkus, MW; Kelly, PF; Garcia, JV;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Internal Med, Div Infect Dis, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Med, Div Infect Dis, Dallas, TX 75390 USA St Jude Childrens Res Hosp, Dept Hematol, Memphis, TN 38105 USA St Jude Childrens Res Hosp Memphis TN USA 38105 ol, Memphis, TN 38105 USA
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 9, volume: 12, anno: 2001,
pagine: 1079 - 1089
SICI:
1043-0342(200106)12:9<1079:LEONDC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEMATOPOIETIC-CELLS; HUMAN CORD-BLOOD; SCID-REPOPULATING CELLS; EFFICIENT GENE-TRANSFER; LENTIVIRAL VECTORS; IN-VIVO; BONE-MARROW; STABLE TRANSDUCTION; NONDIVIDING CELLS; HIGHLY EFFICIENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia, JV Univ Texas, SW Med Ctr, Dept Internal Med, Div Infect Dis, Y9-206,5323 Harry Hines Blvd, Dallas, TX 75390 USA Univ Texas Y9-206,5323 HarryHines Blvd Dallas TX USA 75390 USA
Citazione:
J. Gatlin et al., "Long-term engraftment of nonobese diabetic/severe combined immunodeficientmice with human CD34(+) cells transduced by a self-inactivating human immunodeficiency virus type 1 vector", HUM GENE TH, 12(9), 2001, pp. 1079-1089

Abstract

Human hematopoietic cells with in vivo repopulating potential hold much promise as a target for corrective gene transfer for numerous inherited or acquired hematopoietic disorders. Here we demonstrate long-term hematopoieticreconstitution of nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice with human CD34(+) cells transduced by an HIV-1-based self-inactivating (SIN) vector encoding the enhanced green fluorescent protein (EGFP). Human umbilical cord CD34(+) cells were transduced (up to 76%) at a low multiplicity of infection (MOI of 5) in the absence of cytokine prestimulation. Introduction of transduced hCD34(+) cells into irradiated recipients resulted in multilineage engraftment and stable transgene expression for 18 weeks posttransplantation. Bone marrow from transplanted mice contained up to50% hCD45(+) cells and up to 63% hCD45(+)/EGFP(+) cells. Analysis of extramedullar splenic reconstitution showed up to 13% hCD45(+) cells and up to 41% hCD45(+)/EGFP(+) cells. Analysis of human progenitor cells isolated frombone marrow of recipient animals showed equivalent percentages of EGFP(+) colony-forming cells (CFCs) by fluorescence microscopy and by PCR analysis of provirus sequences, indicating minimal transgene silencing in vivo. These findings demonstrate the utility of lentivirus-based SIN vectors for hematopoietic stem cell gene transfer and provide strong support for their future clinical evaluation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 02:57:26