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Titolo:
Peroxisomes exist in growth cones and move anterogradely and retrogradely in neurites of PC12D cells
Autore:
Ishikawa, T; Kawai, C; Sano, M; Minatogawa, Y;
Indirizzi:
Kawasaki Med Sch, Dept Biochem, Okayama 7010192, Japan Kawasaki Med Sch Okayama Japan 7010192 t Biochem, Okayama 7010192, Japan Kyoto Prefectural Univ Med, Dept Biol, Kyoto 6038334, Japan Kyoto Prefectural Univ Med Kyoto Japan 6038334 iol, Kyoto 6038334, Japan
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 266, anno: 2001,
pagine: 260 - 269
SICI:
0014-4827(20010610)266:2<260:PEIGCA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL NERVOUS-SYSTEM; AXONAL-TRANSPORT; ENDOPLASMIC-RETICULUM; MONOMERIC MOTOR; F-ACTIN; PROTEIN; KINESIN; MICROTUBULES; MOTILITY; MITOCHONDRIA;
Keywords:
peroxisome; neurite; growth cone; motility; GFP; anterograde transport; retrograde transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Ishikawa, T Kawasaki Med Sch, Dept Biochem, 577 Matsushima, Okayama 7010192, Japan Kawasaki Med Sch 577 Matsushima Okayama Japan 7010192 , Japan
Citazione:
T. Ishikawa et al., "Peroxisomes exist in growth cones and move anterogradely and retrogradely in neurites of PC12D cells", EXP CELL RE, 266(2), 2001, pp. 260-269

Abstract

Localization and movement of peroxisomes have been investigated in neurites of a subline of PC12 pheochromocytoma cells (PC12D cells). The cells weretransfected with a construct encoding the green fluorescent protein and bearing the C-terminal peroxisomal targeting signal 1 SKL motif (-Ser-Lys-Leu-COOH). Peroxisomes were detected as green punctate fluorescent signals. Many peroxisomes mere observed in neurites of PC12D cells, especially in neural terminal-like structures, growth cones, varicosities, and branch points. Growth cones containing many peroxisomes were active, since they extended several long filopodias. Existence of peroxisomes in growth cones and neuronal terminal-like structures suggests that peroxisomes might have some rolein neuronal extension and nerve terminal functioning. Peroxisomal motilitywas analyzed by time-lapse imaging using a fluorescence microscope at 25 degreesC. Peroxisomes were transported bidirectionally in neurites, i.e., through anterograde and retrograde transport. This result suggests that peroxisomes move to growth cones and neural terminals from the PC12D cell body, play some role in these parts, and go back to cell body. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:41:03