Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Comparison between hypothermia and glutamate antagonism treatments on the immediate outcome of perinatal asphyxia
Autore:
Engidawork, E; Loidl, F; Chen, Y; Kohlhauser, C; Stoeckler, S; DellAnna, E; Lubec, B; Lubec, G; Goiny, M; Gross, J; Andersson, K; Herrera-Marschitz, M;
Indirizzi:
Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 acol, S-17177 Stockholm, Sweden Karolinska Inst, Dept Internal Med, Stockholm, Sweden Karolinska Inst Stockholm Sweden , Dept Internal Med, Stockholm, Sweden Univ Vienna, Dept Pediat, Vienna, Austria Univ Vienna Vienna AustriaUniv Vienna, Dept Pediat, Vienna, Austria Univ Udine, Dept Exptl Clin Pathol & Med, I-33100 Udine, Italy Univ UdineUdine Italy I-33100 l Clin Pathol & Med, I-33100 Udine, Italy Humboldt Univ, Charite Hosp, Dept Otorhinolaryngol, Berlin, Germany Humboldt Univ Berlin Germany sp, Dept Otorhinolaryngol, Berlin, Germany Univ Chile, Fac Med, ICBM, Programme Mol & Clin Pharmacol, Santiago, ChileUniv Chile Santiago Chile ogramme Mol & Clin Pharmacol, Santiago, Chile
Titolo Testata:
EXPERIMENTAL BRAIN RESEARCH
fascicolo: 3, volume: 138, anno: 2001,
pagine: 375 - 383
SICI:
0014-4819(200106)138:3<375:CBHAGA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISCHEMIC BRAIN INJURY; PROTEIN-KINASE-C; NEUROTRANSMITTER RELEASE; CEREBRAL HYPOTHERMIA; NEONATAL ASPHYXIA; NEURONAL DAMAGE; RAT; ACIDOSIS; DEATH; MICRODIALYSIS;
Keywords:
asphyxia; neonatal; hypothermia; glutamate; antagonism; microdialysis; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Herrera-Marschitz, M Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 lm, Sweden
Citazione:
E. Engidawork et al., "Comparison between hypothermia and glutamate antagonism treatments on the immediate outcome of perinatal asphyxia", EXP BRAIN R, 138(3), 2001, pp. 375-383

Abstract

This study investigated the influence of temperature or glutamate antagonism on the immediate outcome of perinatal asphyxia. Perinatal asphyxia was produced by water immersion of fetus-containing uterus horns removed by cesarean section from ready to deliver rats. The uterus horns were kept in a water bath for different time periods, before the pups were delivered and stimulated to breathe. After delivery, the pups were assessed for behavior andfor systemic glutamate, aspartate, lactate and pyruvate levels measured with in vivo microdialysis, or ex vivo for energy-rich phosphates, including adenosine triphosphate (ATP), in brain, heart and kidney. In a series of experiments, asphyxia was initiated in a water bath at 37 degreesC, before the pup-containing uterus horns were moved for different time intervals to a 15 degreesC bath. In another series of experiments, the mothers were treated with N-methyl-D-aspartate (NMDA) antagonist, dizocilpine (MK-801), or alpha -amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) antagonist, 2,3-dihydroxy-6-nitro-7-sulfamoyl benzo(f) quinoxaline (NBQX) 1 h before hysterectomy and asphyxia at 37 degreesC. The rate of survival rapidly decreased following exposure to more than 16 min of asphyxia, and no survival could be observed after 22 min of asphyxia. An LD50 was estimated to occur at similar to 19 min of asphyxia. The outcome was paralleled by a decrease in ATP in kidney, followed by a decrease in heart and brain. A maximal decreasein ATP was observed after 20 min of asphyxia in all tissues. Systemic microdialysis revealed that glutamate, aspartate and pyruvate levels were increased with a peak after 5 min of asphyxia. In contrast, lactate levels increased along with the length of the insult. Survival was increased when the pup-containing uterus horns were moved from a 37 degreesC to a 15 degreesC bath, at 15 min of asphyxia (the LD50 was thus increased to 30 min). If the shift occurred at 10 or 5 min of asphyxia, the LD50 increased to 80 or 110 min, respectively. The effect of glutamate antagonism was minor compared tohypothermia; the best effect (an increase in the LD50 to similar to 22 min) was observed after combining AMPA and NMDA antagonists.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:36:57