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Titolo:
Identification of committed NK cell progenitors in adult murine bone marrow
Autore:
Rosmaraki, EE; Douagi, I; Roth, C; Colucci, F; Cumano, A; Di Santo, JP;
Indirizzi:
Inst Pasteur, Unite Cytokines & Dev Lymphoide, F-75742 Paris, France Inst Pasteur Paris France F-75742 & Dev Lymphoide, F-75742 Paris, France Inst Pasteur, Unite Dev Lymphocytes, Paris, France Inst Pasteur Paris France Pasteur, Unite Dev Lymphocytes, Paris, France
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 6, volume: 31, anno: 2001,
pagine: 1900 - 1909
SICI:
0014-2980(200106)31:6<1900:IOCNCP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; HEMATOPOIETIC STEM-CELLS; T-CELL; FETAL THYMUS; TRANSCRIPTION FACTOR; LINEAGE COMMITMENT; IN-VITRO; MOUSE; DIFFERENTIATION; MICE;
Keywords:
cytolysis; hematopoiesis; interleukin; lymphocyte; precursor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Di Santo, JP Inst Pasteur, Unite Cytokines & Dev Lymphoide, 25 Rue Dr Roux, F-75742 Paris, France Inst Pasteur 25 Rue Dr Roux Paris France F-75742 ris, France
Citazione:
E.E. Rosmaraki et al., "Identification of committed NK cell progenitors in adult murine bone marrow", EUR J IMMUN, 31(6), 2001, pp. 1900-1909

Abstract

Natural killer (NK) cells play important roles in innate immunity by lysing tumor and virally infected cells and by producing cytokines including interferon-gamma. While NK cell progenitors have been described in the fetal thymus, NK cell generation from hematopoietic stem cells (HSC) in the bone marrow (BM) occurs throughout life, and in athymic mice and humans. Interleukin (IL)-15 promotes NK development in vitro and is essential for the generation of normal numbers of NK cells in vivo. By characterizing BM cells expressing IL-15 receptor components, we found marked heterogeneity within theIL-2 receptor beta chain(+) (CD122(+)) subset, which included cells uniquely committed to the NK lineage. These CD122(+) (NK) under bar cell (p) under bar recursors (NKP) are negative for markers used to identify mature NK cells, including NK1.1, DX5 and members of Ly-49 family, and fail to demonstrate natural cytotoxicity against susceptible target cells. In vitro culture of NKP generates mature lytic NK1.1(+) cells at high frequencies, while they do not give rise to T, B, myeloid or erythroid cells under appropriate conditions. NKP lack transcripts associated with early B and T cell differentiation (pT alpha, lambda5 and CD3 epsilon), but express a group of genes (IL-15R alpha, Id2, GATA-3 and Ets-1) and the 2B4 marker, which may define NK cell committment. We propose that NKP represent the earliest adult BM precursor uniquely restricted to the NK cell lineage.

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Documento generato il 23/09/20 alle ore 22:17:45