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Titolo:
Co-localization of vesicles and P/Q Ca2+-channels explains the preferential distribution of exocytotic active zones in neurites emitted by bovine chromaffin cells
Autore:
Gil, A; Viniegra, S; Neco, P; Gutierrez, LM;
Indirizzi:
Univ Miguel Hernandez, Inst Neurociencias, CSIC, Ctr Mixto, E-03550 Alicante, Spain Univ Miguel Hernandez Alicante Spain E-03550 to, E-03550 Alicante, Spain
Titolo Testata:
EUROPEAN JOURNAL OF CELL BIOLOGY
fascicolo: 5, volume: 80, anno: 2001,
pagine: 358 - 365
SICI:
0171-9335(200105)80:5<358:COVAPC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOPAMINE-BETA-HYDROXYLASE; CALCIUM CHANNELS; CATECHOLAMINE SECRETION; OMEGA-CONOTOXIN; CA2+ CHANNELS; RELEASE; SNAP-25; SITES; COMPONENTS; CULTURE;
Keywords:
exocytosis; active zones; calcium channel subtypes; chromaffin cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Gutierrez, LM Univ Miguel Hernandez, Inst Neurociencias, CSIC, Ctr Mixto, Campus San Juan, E-03550 Alicante, Spain Univ Miguel Hernandez Campus San Juan Alicante Spain E-03550
Citazione:
A. Gil et al., "Co-localization of vesicles and P/Q Ca2+-channels explains the preferential distribution of exocytotic active zones in neurites emitted by bovine chromaffin cells", EUR J CELL, 80(5), 2001, pp. 358-365

Abstract

We have taken advantage of the differences between the preferential localization of secretion in the terminals of neurite-emitting bovine chromaffin cells in contrast with the random distribution secretion in spherical cellsto study the possible molecular factors determining such localization by using immunofluorescence and confocal microscopy techniques. By analyzing the distribution of dopamine P-hydroxylase present in the membrane of chromaffin granules, we found that vesicles migrate and accumulate in dense packages in the terminals of neurite processes. Neither members of the fusion core complex such las SNAP-25, nor nicotinic receptors are preferentially located in the terminals as would be expected from elements defining sites of release, thereby suggesting the presence of additional factors. Interestingly, we observed a preferential distribution of the P/Q subtype of Ca2+ channels in these neurite terminals and co-localization with vesicles present inthese structures, in sharp contrast with the overall distribution of the Lsubtype channels, Using the same immunofluorescence techniques we were unable to detect N-type calcium channels. Zn addition, omega -agatoxin IVA wasable to block 70% of the exocytotic release occurring into the neurites, whereas L-type blockers had a weak effect. Taken together our results strongly indicate that the co-localization of vesicles and clusters of P/Q Ca2+ channels may explain the precise localization of exocytotic sites in the terminals of neurite-emitting chromaffin cells, whereas the distribution of secretory sites in round cells may arise front the random presence of these factors as indicated by their partial co-localization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:17:00