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Titolo:
EXISTENCE OF LIPOPROTEIN-LIPASE IN HUMAN SARCOMAS AND CARCINOMAS
Autore:
SAKAYAMA K; MASUNO H; MIYAZAKI T; OKUMURA H; SHIBATA T; OKUDA H;
Indirizzi:
EHIME UNIV,SCH MED,DEPT MED BIOCHEM SHIGENOBU EHIME 79102 JAPAN EHIME UNIV,SCH MED,DEPT MED BIOCHEM SHIGENOBU EHIME 79102 JAPAN EHIME UNIV,SCH MED,DEPT ORTHOPAED SURG SHIGENOBU EHIME 79102 JAPAN EHIME UNIV,SCH MED,DEPT PATHOL 2 SHIGENOBU EHIME 79102 JAPAN
Titolo Testata:
Japanese journal of cancer research
fascicolo: 5, volume: 85, anno: 1994,
pagine: 515 - 521
SICI:
0910-5050(1994)85:5<515:EOLIHS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL NUCLEAR ANTIGEN; ASCITES-TUMOR; PROLIFERATION; MACROPHAGES; ADIPOCYTES; MEMBRANES; SECRETION; TISSUES; ACIDS; MICE;
Keywords:
LIPOPROTEIN LIPASE; SARCOMA; CARCINOMA; PROLIFERATING CELL NUCLEAR ANTIGEN; HUMAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
K. Sakayama et al., "EXISTENCE OF LIPOPROTEIN-LIPASE IN HUMAN SARCOMAS AND CARCINOMAS", Japanese journal of cancer research, 85(5), 1994, pp. 515-521

Abstract

Aqueous extracts of acetone/ether powders of surgically obtained specimens of human tumors hydrolyzed H-3-labeled triolein in a dose-dependent manner. The lipolytic activity in these extracts was inhibited by anti-lipoprotein lipase (LPL) IgG dose-dependently, 25 mu g of anti-LPL Ige causing 95% inhibition of the activity. Thus, LPL accounts for most of the lipolytic activity in extracts of acetone/ether powders of the tumors. All sarcomas and carcinomas examined contained LPL activity. Western blotting showed that they gave a band corresponding to thatof human adipose tissue LPL (M(r)=57,000). Immunocytochemical studiesshowed that LPL was present in cultured human osteosarcoma cells and distributed throughout the cells. We determined the proliferating cellnuclear antigen (PCNA)-labeling index as an indicator of the proliferative activity of tumor cells and measured LPL activity in extracts oftumors in areas corresponding to those used for determining the PCNA-labeling index. In malignant fibrous histiocytomas, the PCNA labeling index in area a, which corresponds to the subcapsular region, was higher than that in area b, which corresponds to the central region. The LPL activity in area a was 10 times that in area b. In rectal cancer, the index in area c, which corresponds to the subserosal region, was higher than that in area d, which corresponds to the submucosal region. The LPL activity in area c was 1.9 times that in area d. These findings indicate heterogeneity in the distributions of LPL activity within tumors and higher levels of LPL activity in tumors that are proliferating actively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 02:20:18