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Titolo:
Insulin-like growth factor binding proteins in the bovine anterior pituitary
Autore:
Roberts, AJ; Funston, RN; Moss, GE;
Indirizzi:
USDA ARS, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA USDAARS Clay Ctr NE USA 68933 Meat Anim Res Ctr, Clay Ctr, NE 68933 USA Univ Wyoming, Dept Anim Sci, Laramie, WY 82071 USA Univ Wyoming Laramie WY USA 82071 g, Dept Anim Sci, Laramie, WY 82071 USA
Titolo Testata:
ENDOCRINE
fascicolo: 3, volume: 14, anno: 2001,
pagine: 399 - 406
SICI:
1355-008X(200104)14:3<399:IGFBPI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
I IGF-I; LUTEINIZING-HORMONE; FOLLICULAR-FLUID; SERUM; RAT; CELLS; EWES; HYPOTHALAMUS; EXPRESSION; ESTRADIOL;
Keywords:
bovine; pituitary; insulin-like growth factor binding proteins; luteinizing hormone-releasing hormone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Roberts, AJ USDA ARS, Roman L Hruska US Meat Anim Res Ctr, State Spur 18D,POB 166, Clay Ctr, NE 68933 USA USDA ARS State Spur 18D,POB 166 Clay Ctr NEUSA 68933 8933 USA
Citazione:
A.J. Roberts et al., "Insulin-like growth factor binding proteins in the bovine anterior pituitary", ENDOCRINE, 14(3), 2001, pp. 399-406

Abstract

Insulin-like growth factor binding proteins (IGFBPs) were characterized inbovine anterior pituitary tissue, pituitary conditioned media, and serum collected during the preovulatory and early luteal phases of the estrous cycle. Effects of in vitro treatments of pituitaries with luteinizing hormone-releasing hormone (LHRH), estradiol, and progesterone on IGFBP secretion were also evaluated. Predominant IGFBPs detected in anterior pituitary tissueby immunoprecipitation, ligand blotting, and Northern blotting were IGFBP-5 (29 kDa), IGFBP-2 (32 kDa), and IGFBP-3 (36 and 39 kDa doublet). Conditioned culture media contained IGFBP-5, a slightly larger form of IGFBP-2 (33 kDa), the 36- and 39-kDa forms of IGFBP-3, and a more extensively glycosylated form of IGFBP-3 (44 kDa). In serum, IGFBP-5 was not readily detected, and IGFBP-3 (40- and 44-kDa doublet) and IGFBP-2 (34 kDa) were larger than in pituitary tissue. Levels of IGFBP-2, -3, and -5 in pituitary tissue decreased during the preovulatory period and were lowest in the early luteal phase. Treatment with LHRH increased IGFBP-2 levels in media twofold. Estradiol or progesterone did not alter IGFBP secretion in vitro. Predominant IGFBPs produced and released by anterior pituitary tissue were IGFBP-2, -3 and -5. The activity of IGFBPs fluctuates in the pituitary in association with changes in stage of estrous cycle, implicating IGFBPs as potential regulators of gonadotrope function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 00:32:51