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Titolo:
Effect of 1 alpha,25-dihydroxyvitamin D-3 and 24R,25-dihydroxyvitamin D-3 on metalloproteinase activity and cell maturation in growth plate cartilagein vivo
Autore:
Dean, DD; Boyan, BD; Schwartz, Z; Muniz, OE; Carreno, MR; Maeda, S; Howell, DS;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Orthopaed, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 Orthopaed, San Antonio, TX 78229 USA Univ Texas, Hlth Sci Ctr, Dept Periodont, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 Periodont, San Antonio, TX 78229 USA Univ Texas, Hlth Sci Ctr, Dept Biochem, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 pt Biochem, San Antonio, TX 78229 USA Hebrew Univ Jerusalem, Hadassah Fac Dent Med, Dept Periodont, Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel ept Periodont, Jerusalem, Israel GRECC, Miami VA Med Ctr, Arthrit Res Lab, Miami, FL USA GRECC Miami FL USA ECC, Miami VA Med Ctr, Arthrit Res Lab, Miami, FL USA Univ Miami, Sch Med, Dept Med, Miami, FL USA Univ Miami Miami FL USAUniv Miami, Sch Med, Dept Med, Miami, FL USA
Titolo Testata:
ENDOCRINE
fascicolo: 3, volume: 14, anno: 2001,
pagine: 311 - 323
SICI:
1355-008X(200104)14:3<311:EO1ADA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAMIN-D METABOLITES; RESTING ZONE CHONDROCYTES; HUMAN ARTICULAR-CARTILAGE; AGGRECAN INTERGLOBULAR DOMAIN; ENDOCHONDRAL BONE-FORMATION; EPIPHYSEAL CARTILAGE; MATRIX METALLOPROTEINASE-2; INTERSTITIAL COLLAGENASE; NEUTROPHIL COLLAGENASE; EXTRACELLULAR-MATRIX;
Keywords:
endochondral ossification; 1 alpha,25-dihydroxyvitamin D-3; 24R,25-dihydroxyvitamin D-3; metalloproteinase; tissue inhibitor of metalloproteinases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
84
Recensione:
Indirizzi per estratti:
Indirizzo: Dean, DD Univ Texas, Hlth Sci Ctr, Dept Orthopaed, Mail Code 7774,7703 Floyd Curl Dr, San Antonio, TX 78229 USA Univ Texas Mail Code 7774,7703 Floyd Curl Dr San Antonio TX USA 78229
Citazione:
D.D. Dean et al., "Effect of 1 alpha,25-dihydroxyvitamin D-3 and 24R,25-dihydroxyvitamin D-3 on metalloproteinase activity and cell maturation in growth plate cartilagein vivo", ENDOCRINE, 14(3), 2001, pp. 311-323

Abstract

Recent studies indicate that 1 alpha ,25-dihydroxyvitamin D-3 (1(alpha),25[OH](2)D-3) and 24R,25-dihydroxyvitamim D-3 (24R,25[OH](2)D-3) differentially regulate proliferation, differentiation, and matrix synthesis of growth plate chondrocytes. To determine whether both metabolites play the same or different roles in vivo, we used the vitamin D-deficient rat as a model. Rickets was induced and then reversed by administering a single dose of ergocalciferol, 1 alpha ,25 (OH)(2)D-3, or 24R,25 (OH)(2)D-3 and euthanizing theanimals after 4, 24, 48, or 72 h. Growth plates were either processed for histology and histomorphometry or extracted with buffered guanidine-HCl. Neutral metalloproteinase activity in the extracts was measured by use of aggrecan-containing beads, and collagenase activity was determined by use of radioactive type I collagen. The levels of tissue inhibitor of metalloproteinases (TIMP) and plasminogen activator were also determined. The morphologyof the growth plate varied as a function of treatment. While 24R,25(OH)(2)D-3 appeared to affect cell maturation and 1 alpha ,25(OH)(2)D-3 appeared to affect terminal differentiation and calcification, response to ergocalciferol was indicative of the combined responses to the individual metabolites. Enzyme activity was regulated in a differential manner. Treatment with ergocalciferol produced a rapid decline in both neutral metalloproteinase andcollagenase activities that was statistically significant by 4 h. By contrast, 1 alpha ,25(OH)(2)D-3 had no effect on neutral metalloproteinase activity but caused a significant decrease in both active and total collagenase activity by 4 h, while 24R,25(OH)(2)D-3 decreased neutral metailoproteinaseactivity by 48 h and had no effect on collagenase activity. Ergocalciferolhad no effect on TIMP levels at any time examined, whereas 1 alpha ,25(OH)(2)D-3 caused an increase at 48 and 72 h and 24R,25(OH)(2)D-3 completely blocked TIMP production at 4 and 24 h. By contrast, plasminogen activator activity by ergocalciferol was decreased at 4 h, increased by 1 alpha ,25(OH)(2)D-3 at 4 and 24 h, and decreased by 24R,25(OH)(2)D-3 at all time points examined. These in vivo results confirm our previous cell culture observations showing that growth plate chondrocytes are differentially regulated by 1alpha ,25(OH)(2)D-3 and 24R,25(OH)(2)D-3. Moreover, they show definitivelythat these two vitamin D metabolites play distinct roles not only in regulating neutral metalloproteinase and collagenase activities in growth plate cartilage but in cell maturation and calcification of this tissue in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:38:50