Catalogo Articoli (Spogli Riviste)

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Titolo:
Inhibition of renal permeability towards albumin: A new function of apolipoproteins with possible pathogenetic relevance in focal glomerulosclerosis
Autore:
Candiano, G; Musante, L; Zennaro, C; Bruschi, M; Carraro, M; Artero, M; Gusmano, R; Ginevri, F; Perfumo, F; Ghiggeri, G;
Indirizzi:
G Gasslini Children Hosp, Nephrol Sect, Nephrol Unit, I-16148 Genoa, ItalyG Gasslini Children Hosp Genoa Italy I-16148 Unit, I-16148 Genoa, Italy G Gasslini Children Hosp, Lab Nephrol, I-16148 Genoa, Italy G Gasslini Children Hosp Genoa Italy I-16148 phrol, I-16148 Genoa, Italy Univ Trieste, Inst Clin Med, I-34127 Trieste, Italy Univ Trieste TriesteItaly I-34127 Inst Clin Med, I-34127 Trieste, Italy
Titolo Testata:
ELECTROPHORESIS
fascicolo: 9, volume: 22, anno: 2001,
pagine: 1819 - 1825
SICI:
0173-0835(200105)22:9<1819:IORPTA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEGMENTAL GLOMERULOSCLEROSIS; NEPHROTIC SYNDROME; A-IV; PLASMA; LIPOPROTEIN; ELECTROPHORESIS; PROTEINS; SP-40,40; DISEASE; NIDDM;
Keywords:
proteinuria; atherosclerosis; focal glomerulosclerosis; apolipoproteins; permeability factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Ghiggeri, G G Gasslini Children Hosp, Nephrol Sect, Nephrol Unit, I-16148 Genoa, Italy G Gasslini Children Hosp Genoa Italy I-16148 48 Genoa, Italy
Citazione:
G. Candiano et al., "Inhibition of renal permeability towards albumin: A new function of apolipoproteins with possible pathogenetic relevance in focal glomerulosclerosis", ELECTROPHOR, 22(9), 2001, pp. 1819-1825

Abstract

Focal segmental glomerulosclerosis (FSGS) is a degenerative renal disease characterized by the accumulation of extracellular matrix and lipids withinthe glomerular tuft. It has been proposed that an abnormal renal permeabilization towards proteins induced by a putative plasma factor is, in some way, involved in the pathogenesis of the disease. In this paper, we measured the plasma permeability activity (P-a/b) in several sera of patients with FSGS and found a mean activity of 0.82 +/- 0.03 which means a marked increase compared to a mean P-a/b of 0.16 +/- 0.03 in normal controls. Coincubation of FSGS and normal serum reduced the permeability activity within the normal range; normal serum added to the incubation medium after the glomeruli had already been exposed to the FSGS serum had no effect, suggesting the presence of inhibitory substances with a direct effect on a circulating substrate. Finally, the antipermeability activity was retained when heated to 60degreesC but not to 100 degreesC. By serial fractionations of normal serumand reported activity measurements at each step, five natural occurring inhibitors of albumin permeabilization were purified and characterized by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS), as components of apolipoproteins (apo) (apo E-2 and E-4, apo L, the high M-r apo J and a 28 kDa fragment of apo A-IV). Coincubation of each apolipoproteinwith FSGS serum inhibited permeability, but only apo J and apo E-2 and E-4Were found to be crucial for the process. In conclusion, we have purified from normal serum five inhibitors of permeability induced by FSGS serum, all corresponding to apolipoproteins. An imbalance between permeability factors and apolipoproteins may play a pathogenetic role in FSGS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:03:06