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Titolo:
Human proteome enhancement: High-recovery method and improved two-dimensional map of colostral fat globule membrane proteins
Autore:
Quaranta, S; Giuffrida, MG; Cavaletto, M; Giunta, C; Godovac-Zimmermann, J; Canas, B; Fabris, C; Bertino, E; Mombro, M; Conti, A;
Indirizzi:
CNR, Ctr Studi Alimentaz Anim, I-10010 Colleretto Giacosa, Torino, Italy CNR Colleretto Giacosa Torino Italy I-10010 eretto Giacosa, Torino, Italy Univ Piemonte Orientale, Dipartimento Sci & Technol Avanzate, Alessandria,Italy Univ Piemonte Orientale Alessandria Italy l Avanzate, Alessandria,Italy Univ Turin, Dipartimento Biol Anim & Uomo, I-10124 Turin, Italy Univ Turin Turin Italy I-10124 to Biol Anim & Uomo, I-10124 Turin, Italy Univ Coll London, Ctr Mol Med, London, England Univ Coll London London England ll London, Ctr Mol Med, London, England Univ Turin, Cattedra Neonatol, I-10124 Turin, Italy Univ Turin Turin Italy I-10124 , Cattedra Neonatol, I-10124 Turin, Italy
Titolo Testata:
ELECTROPHORESIS
fascicolo: 9, volume: 22, anno: 2001,
pagine: 1810 - 1818
SICI:
0173-0835(200105)22:9<1810:HPEHMA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
2-DIMENSIONAL ELECTROPHORETIC ANALYSIS; IMMOBILIZED PH GRADIENTS; HUMAN-MILK; BREAST CARCINOMAS; CELL-ADHESION; CDNA CLONING; ANTIGEN BA46; IDENTIFICATION; GLYCOPROTEINS; LACTADHERIN;
Keywords:
human milk fat globule membrane proteins bidimensional electrophoresis; identification; mass spectrometry; microsequencing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Conti, A CNR, Ctr Studi Alimentaz Anim, Bioind Pk,Via Ribes 5, I-10010 Colleretto Giacosa, Torino, Italy CNR Bioind Pk,Via Ribes 5 Colleretto GiacosaTorino Italy I-10010
Citazione:
S. Quaranta et al., "Human proteome enhancement: High-recovery method and improved two-dimensional map of colostral fat globule membrane proteins", ELECTROPHOR, 22(9), 2001, pp. 1810-1818

Abstract

The human milk fat globule membrane protein composition is still largely unknown, although it counts for 2-4% of the total milk protein content and contains several important biologically active components. The aim of this work was to create a two-dimensional electrophoresis (2-DE) map of the humanmilk fat globule membrane proteins, both integral and membrane-associated,and to identify and characterize as many protein components as possible. Anew protocol for the solubilization and extraction of the human milk fat globule membrane proteins with a double extraction procedure is presented, and the results compared with the extraction methods reported in the literature. The proteins were separated, in the first dimension, by isoelectric focusing (IEF) in the pH range 3-10 on strips of 13 cm length and, in the second dimension, by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) on 11.5% T homogeneous gels. A reproducible 2-DE map of integral and membrane-associated proteins was obtained and the first 23 spots, representing the major components, were identified by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometric analysis and/or by amino acid sequencing.

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Documento generato il 07/07/20 alle ore 11:09:13