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Titolo:
Roles of retinoic acid receptors in early embryonic morphogenesis and hindbrain patterning
Autore:
Wendling, O; Ghyselinck, NB; Chambon, P; Mark, M;
Indirizzi:
ULP, Coll France, INSERM, CNRS,IGBMC, F-67404 Illkirch, France ULP Illkirch France F-67404 INSERM, CNRS,IGBMC, F-67404 Illkirch, France
Titolo Testata:
DEVELOPMENT
fascicolo: 11, volume: 128, anno: 2001,
pagine: 2031 - 2038
SICI:
0950-1991(200106)128:11<2031:RORARI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEGMENT-SPECIFIC EXPRESSION; HOMEOBOX-CONTAINING GENE; VITAMIN-A-DEFICIENCY; RAR DOUBLE MUTANTS; MOUSE DEVELOPMENT; NERVOUS-SYSTEM; NUCLEAR RECEPTORS; CAUDAL HINDBRAIN; ALPHA; BETA;
Keywords:
nuclear receptors; rhombomeres; hox genes; kreisler; embryo culture; mouse; vitamin A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Mark, M ULP, Coll France, INSERM, CNRS,IGBMC, BP 163,CU Strasbourg, F-67404 Illkirch, France ULP BP 163,CU Strasbourg Illkirch France F-67404 Illkirch, France
Citazione:
O. Wendling et al., "Roles of retinoic acid receptors in early embryonic morphogenesis and hindbrain patterning", DEVELOPMENT, 128(11), 2001, pp. 2031-2038

Abstract

Mutants mice carrying targeted inactivations of both retinoic acid receptor (RAR) alpha and RAR gamma (A alpha /A gamma mutants) were analyzed at different embryonic stages, in order to establish the timing of appearance of defects that we previously observed during the fetal period. We show that embryonic day (E)9.5 A alpha /A gamma embryos display severe malformations, similar to those already described in retinaldehyde dehydrogenase 2 null mutants. These malformations reflect early roles of retinoic acid signaling in axial rotation, segmentation and closure of the hindbrain; formation of otocysts, pharyngeal arches and forelimb buds; and in the closure of the primitive gut. The hindbrain of E8.5 A alpha /A gamma embryos shows a posterior expansion of rhombomere 3 and 4 (R3 and R4) markers, but fails to expresskreisler, a normal marker of R5 and R6. This abnormal hindbrain phenotype is strikingly different from that of embryos lacking RAR alpha and RAR beta(A alpha /A beta mutants), in which we have previously shown that the territory corresponding to R5 and R6 is markedly enlarged. Administration of a pan-RAB antagonist at E8.0 to wild-type embryos cultured in vitro results in an A alpha /A beta -like hindbrain phenotype, whereas an earlier treatment at E7.0 yields an A alpha /A gamma -like phenotype, Altogether, our data suggest that RAR alpha and/or RAR gamma transduce the RA signal that is required first to specify the prospective R5/R6 territory, whereas RAR beta issubsequently involved in setting up the caudal boundary of this territory.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:15:31