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Titolo:
Molecular cloning of gerbil interleukin 12 and its expression as a bioactive single-chain protein
Autore:
Gaucher, D; Chadee, K;
Indirizzi:
McGill Univ, Inst Parasitol, St Anne De Bellevue, PQ H9X 3V9, Canada McGill Univ St Anne De Bellevue PQ Canada H9X 3V9 vue, PQ H9X 3V9, Canada
Titolo Testata:
CYTOKINE
fascicolo: 3, volume: 14, anno: 2001,
pagine: 177 - 183
SICI:
1043-4666(20010507)14:3<177:MCOGI1>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL STIMULATORY FACTOR; IL-12 P40 HOMODIMER; HETERODIMERIC CYTOKINE; MERIONES-UNGUICULATUS; IN-VIVO; SUBUNIT;
Keywords:
cloning; expression; gerbil; interleukin 12; single-chain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Chadee, K McGill Univ, Inst Parasitol, 21-111 Lakeshore Rd,Ste Anne Bellevue,Macdonald C, St Anne De Bellevue, PQ H9X 3V9, Canada McGill Univ 21-111 Lakeshore Rd,Ste Anne Bellevue,Macdonald C St Anne De Bellevue PQ Canada H9X 3V9
Citazione:
D. Gaucher e K. Chadee, "Molecular cloning of gerbil interleukin 12 and its expression as a bioactive single-chain protein", CYTOKINE, 14(3), 2001, pp. 177-183

Abstract

Complementary DNAs coding for gerbil interleukin 12 (IL-12) p40/p35 subunits were cloned by a combination of cross species reverse transcriptase-polymerase chain reaction (RT-PCR) and 3 ' rapid amplification of cDNA ends (RACE) techniques. IL-12 p40/p35 had 79% nucleotide identity and 81% amino acid homology to mouse IL-12 p40/p35. The p40/p35 subunits were expressed as asingle polypeptide separated by a short hinge sequence that allowed for proper folding and assembly. COS-7 cells transfected with DNA encoding the single-chain gerbil IL-12 (pSCjIL12) secreted high levels of the protein, which stimulated proliferation of ConA-activated gerbil spleen lymphoblasts ina dose-dependent manner. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:17:23