Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Expression of interleukin 7 (IL-7) mRNA and protein in the normal adult human liver: Implications for extrathymic T cell development
Autore:
Golden-Mason, L; Kelly, AM; Traynor, O; McEntee, G; Kelly, J; Hegarty, JE; OFarrelly, C;
Indirizzi:
Univ Dublin, St Vincents Hosp, Educ & Res Ctr, Dublin 4, Ireland Univ Dublin Dublin Ireland 4 nts Hosp, Educ & Res Ctr, Dublin 4, Ireland Univ Dublin, St Vincents Hosp, Natl Liver Transplant Unit, Dublin 4, Ireland Univ Dublin Dublin Ireland 4 tl Liver Transplant Unit, Dublin 4, Ireland Dublin Inst Technol, Dublin, Ireland Dublin Inst Technol Dublin IrelandDublin Inst Technol, Dublin, Ireland
Titolo Testata:
CYTOKINE
fascicolo: 3, volume: 14, anno: 2001,
pagine: 143 - 151
SICI:
1043-4666(20010507)14:3<143:EOI7(M>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; RECEPTOR-GAMMA-CHAIN; SEVERE COMBINED IMMUNODEFICIENCY; BONE-MARROW; CYTOKINE SECRETION; GROWTH-FACTOR; LYMPHOCYTES; GENERATION; MICE; DIFFERENTIATION;
Keywords:
RT-PCR; ELISA; cytokine; lymphopoiesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: O'Farrelly, C Univ Dublin, St Vincents Hosp, Educ & Res Ctr, Elm Pk, Dublin 4, Ireland Univ Dublin Elm Pk Dublin Ireland 4 m Pk, Dublin 4, Ireland
Citazione:
L. Golden-Mason et al., "Expression of interleukin 7 (IL-7) mRNA and protein in the normal adult human liver: Implications for extrathymic T cell development", CYTOKINE, 14(3), 2001, pp. 143-151

Abstract

Interleukin-7 (IL-7) has been shown to play an essential role in T-cell development, Recombinase-activating gene CRAG)-1, RAG-2 and pre-TCR-a expression in the normal adult human liver (AHL), together,vith the presence of lymphoid-haematopoietic progenitors, is strong evidence that the AHL supportsT cell maturation. We investigated IL-7 mRNA and protein levels in order to determine whether AHL could support T lymphocyte differentiation. Biopsies were snap frozen, powdered, and RNA/protein extracted, Reverse transcriptase polymerase chain reaction was used to detect IL-7 using primers that amplified 620 base pair (bp) fragments and other smaller transcripts. A sandwich enzyme-linked immunosorbent assay was developed to quantify IL-7 protein in homogenates. The anatomic distribution of IL-7-secreting cells was determined by immunohistochemistry. IL-7-specific product (620 bp) was detected in nine of ten samples, with six also positive for a smaller splice-variant (488 bp), Levels of the 620 bp product were 2.5 times greater than the 488 bp fragment. IL-7 protein was detected in all samples (range 18.47-76.93pg/100 mg tissue). Immunohistochemistry demonstrated IL-7 protein in discrete cells of lymphoid morphology, widely distributed throughout the parenchyma and within portal tracts. Large populations of innate T cells are foundin normal AHL, some of which may differentiate locally, The presence of IL-7 RNA and protein throughout normal hepatic tissue provides evidence that the normal AHL is a suitable microenvironment for T cell differentiation. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:05:04