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Titolo:
Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes
Autore:
Sathiyaa, R; Campbell, T; Vijayan, MM;
Indirizzi:
Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 ol, Waterloo, ON N2L 3G1, Canada
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 2-3, volume: 129, anno: 2001,
pagine: 679 - 685
SICI:
1096-4959(200106)129:2-3<679:CMHMEI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; HEPATIC HEAT-SHOCK-PROTEIN-70; GLUCOCORTICOID RECEPTOR; MESSENGER-RNA; RAINBOW-TROUT; STRESS; HSP90-ALPHA; LIVER; FISH;
Keywords:
cortisol; heat shock protein; HSP90 mRNA; glucocorticoid receptors; fish; rainbow trout; hepatocyte primary cultures; stress; temperature shock;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Vijayan, MM Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 , ON N2L 3G1, Canada
Citazione:
R. Sathiyaa et al., "Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes", COMP BIOC B, 129(2-3), 2001, pp. 679-685

Abstract

The objective of the present study was to understand the role of cortisol in the cellular stress response process in fish. Specifically, our studies addressed whether cortisol exposure modified heat shock protein 90 (HSP90) mRNA expression in rainbow trout (Oncorhynchus mykiss) hepatocytes maintained in primary culture. We also subjected these hepatocytes to heat shock (HS) in order to examine the role of cortisol on MS-induced HSP90 mRNA expression. A cDNA fragment of 500 bp was cloned from trout liver by reverse transcriptase polymerase chain reaction (RT-PCR) with primers designed from theconserved regions of chinook salmon and zebrafish HSP90 cDNAs. The PCR product showed very high homology to chinook salmon (98%), zebrafish (84%) andhuman (77%) HSP90. Heat shock (+6 degreesC) induced transient elevation inHSP90 mRNA in trout hepatocytes, peaking within 10-h post-HS, and remainedelevated over a 24-h period. Cortisol did not modify the unstimulated expression of HSP90 mRNA, whereas the MS-induced HSP90 mRNA expression was attenuated in trout hepatocytes. Our results suggest that elevated plasma cortisol levels modulate the cellular stress response by affecting the transcription of HSP90 in fish. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 00:37:03