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Titolo:
Developmental expression, tissue distribution and hormonal regulation of fish (Sparus aurata) serum retinol-binding protein
Autore:
Funkenstein, B;
Indirizzi:
Natl Inst Oceanog, Israel Oceanog & Limnol Res, IL-31080 Haifa, Israel Natl Inst Oceanog Haifa Israel IL-31080 mnol Res, IL-31080 Haifa, Israel
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 2-3, volume: 129, anno: 2001,
pagine: 613 - 622
SICI:
1096-4959(200106)129:2-3<613:DETDAH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; MESSENGER-RNA; VITAMIN-A; GILTHEAD SEABREAM; 3-DIMENSIONAL STRUCTURE; PLASMA GONADOTROPIN; FETAL DEVELOPMENT; THYROID-HORMONES; SEX STEROIDS; YOLK-SAC;
Keywords:
embryo; estrogen; larva; liver; RNA blot hybridization; RT-PCR; skin; sparidae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Funkenstein, B Natl Inst Oceanog, Israel Oceanog & Limnol Res, POB 8030, IL-31080 Haifa, Israel Natl Inst Oceanog POB 8030 Haifa Israel IL-31080 a, Israel
Citazione:
B. Funkenstein, "Developmental expression, tissue distribution and hormonal regulation of fish (Sparus aurata) serum retinol-binding protein", COMP BIOC B, 129(2-3), 2001, pp. 613-622

Abstract

Retinol-binding protein (RBP) is the specific carrier of retinol in vertebrates and forms a 1:1 complex with transthyretin (TTR). A cDNA encoding serum REP was cloned from liver and 7-day larvae of the marine fish Sparus aurata. The mature protein is 176 amino acids long and shows sequence identityof 77-78%, 56%, 63% and 62% with rainbow trout, Xenopus, chicken and humanREP, respectively. Northern blot analysis of hepatic REP revealed two transcripts: a major one of approximately 1.4-1.5 kb and a minor of approximately 0.7 kb. Distribution of REP mRNA in various tissues was studied by RT-PCR and showed high expression in liver and skin, and low expression in brain, kidney and gill filament (20-35% of the level in liver). REP expression in intestine, pyloric caeca, muscle and pituitary was estimated to be approximately 7-14% of the level in liver. The ontogeny of REP expression in S. aurata was examined in unfertilized eggs, embryos and larvae by using RT-PCRfollowed by hybridization with a specific probe. REP transcript was found in all larval stages studied. Very low levels of REP mRNA were detected in unfertilized eggs and in embryos 8 h after fertilization with a gradual increase at 12 h and 15-16 h post-fertilization. A single injection of estradiol-17 beta to S. aurata immature, bisexual fish or to adult males reduced steady-state levels of hepatic REP by 37 and 25%, respectively. The same treatment induced vitellogenin expression. The present data suggest that in fish, liver is the main site of REP synthesis, but that REP may have an important function in fish skin. REP is expressed early in embryonic developmentand in fish its expression can be down regulated by estrogen. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:21:17