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Titolo:
Entry of thyroid hormones into tilapia oocytes
Autore:
Tagawa, M; Brown, CL;
Indirizzi:
Kyoto Univ, Grad Ssch Agr, Div Appl Biosci, Sakyo Ku, Kyoto 6068502, JapanKyoto Univ Kyoto Japan 6068502 pl Biosci, Sakyo Ku, Kyoto 6068502, Japan Florida Int Univ, Marine Biol Program, Miami, FL 33199 USA Florida Int Univ Miami FL USA 33199 ine Biol Program, Miami, FL 33199 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 2-3, volume: 129, anno: 2001,
pagine: 605 - 611
SICI:
1096-4959(200106)129:2-3<605:EOTHIT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
OREOCHROMIS-MOSSAMBICUS; STRIPED BASS; TRIIODOTHYRONINE; THYROXINE; BROODSTOCK; SURVIVAL; CORTISOL; TELEOSTS; LARVAE; SALMON;
Keywords:
maternal hormone; thyroid hormones; T4; T3; egg; ovarian follicle; tilapia; teleost; reproduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Tagawa, M Kyoto Univ, Grad Ssch Agr, Div Appl Biosci, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Sakyo Ku, Kyoto 6068502, Japan
Citazione:
M. Tagawa e C.L. Brown, "Entry of thyroid hormones into tilapia oocytes", COMP BIOC B, 129(2-3), 2001, pp. 605-611

Abstract

The patterns of entry of thyroid hormones into live tilapia oocytes were examined by incubating ovarian follicles in L-15 medium containing (125)-labeled thyroxine (T4) or 3,5,3'-triiodothyronine (T3). As judged from HPLC profiles, radioactivity in extracts of follicles immersed in T3 was identified to reside in T3, while most of the radioactivity in the extract of T4 immersed follicle was not associated with T4. Radioactivity of T3 immersed follicles reached a constant level after 18 h of incubation. Entry of T3 into the oocytes was non-saturable within the range of 0.5-5000 ng/ml of T3 in the incubation medium, suggesting the absence of specific mechanisms for T3 entry into the oocyte. Presence of female plasma at a level of 20% of incubation medium inhibited the n entry into the oocytes by approximately 80%. When follicles were back-transferred to medium without T3, only 15% of T3 inthe oocyte disappeared within the following 24 h. From our results, we conclude that free T3 seems to enter oocytes freely across the membranes by diffusion, and that T3 in the oocytes may bind to some molecules in the oocyte. However, during egg formation in vivo, contribution of free T3 entry into the oocytes did not seem to be significant when considering the free T3 ratio in female plasma. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:52:58