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Titolo:
Sequence conservation among fish myostatin orthologues and the characterization of two additional cDNA clones from Morone saxatilis and Morone americana
Autore:
Rodgers, BD; Weber, GM;
Indirizzi:
Johns Hopkins Univ, Sch Med, Baltimore, MD 21287 USA Johns Hopkins Univ Baltimore MD USA 21287 ch Med, Baltimore, MD 21287 USA N Carolina State Univ, Dept Zool, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 pt Zool, Raleigh, NC 27695 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 2-3, volume: 129, anno: 2001,
pagine: 597 - 603
SICI:
1096-4959(200106)129:2-3<597:SCAFMO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA SUPERFAMILY MEMBER; SKELETAL-MUSCLE MASS; CATTLE; MUTATIONS; GENE;
Keywords:
growth; development; myostatin; GDF-8; skeletal muscle; aquaculture; Morone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Rodgers, BD Johns Hopkins Univ, Sch Med, 600 N Wolfe St,PK 211, Baltimore,MD 21287 USA Johns Hopkins Univ 600 N Wolfe St,PK 211 Baltimore MD USA 21287
Citazione:
B.D. Rodgers e G.M. Weber, "Sequence conservation among fish myostatin orthologues and the characterization of two additional cDNA clones from Morone saxatilis and Morone americana", COMP BIOC B, 129(2-3), 2001, pp. 597-603

Abstract

Myostatin (MSTN) negatively regulates mammalian skeletal muscle growth anddevelopment by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. MSTN orthologueshave been previously characterized in 12 vertebrate species, including thewhite bass Morone chrysops. Presented herein is the identification and characterization of novel cDNA clones from two additional Morone species: saxatilis (striped bass) and americana (white perch), which were obtained by PCR amplification and subsequent TA-cloning. The predicted amino acid sequence of each cDNA clone contains a putative signal sequence, conserved cysteine residues and a RXXR proteolytic processing site. The different Morone proteins were 97-99% identical to each other and approximately 91, 81, 68 and 67% identical to the tilapia, zebrafish, mammalian and avian proteins, respectively. However, the bioactive domains, which lie downstream of each processing site, were considerably more conserved. They were 99-100% identical within the genus and were approximately 99, 95, 88 and 88% identical to thetilapia, zebrafish, mammalian and avian domains, respectively. This high level of sequence conservation among all known MSTN orthologues suggests that the structure/function relationship of each is equally well conserved among vertebrates. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 01:10:13