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Titolo:
Two subtypes of androgen and progestogen receptors in fish testes
Autore:
Ikeuchi, T; Todo, T; Kobayashi, T; Nagahama, Y;
Indirizzi:
Natl Inst Basic Biol, Reprod Biol Lab, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 ki, Aichi 4448585, Japan Niigata Univ, Fac Sci, Sado Marine Biol Stn, Niigata 9522135, Japan Niigata Univ Niigata Japan 9522135 rine Biol Stn, Niigata 9522135, Japan
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 2-3, volume: 129, anno: 2001,
pagine: 449 - 455
SICI:
1096-4959(200106)129:2-3<449:TSOAAP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EEL ANGUILLA-JAPONICA; MALE JAPANESE EEL; ESTROGEN-RECEPTOR; PROGESTERONE RECEPTORS; MOLECULAR-CLONING; STEROID-HORMONES; CDNA CLONING; EVOLUTION; MATURATION; SPERMATOGENESIS;
Keywords:
androgen receptor; progestogen receptor; subtype; androgen; progestogen; testis; spermatogenesis; eel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Nagahama, Y Natl Inst Basic Biol, Reprod Biol Lab, Okazaki, Aichi 4448585,Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 48585, Japan
Citazione:
T. Ikeuchi et al., "Two subtypes of androgen and progestogen receptors in fish testes", COMP BIOC B, 129(2-3), 2001, pp. 449-455

Abstract

Two subtypes (a and P) of androgen (AR) and progestogen receptors (PR) arepresent in the testis of Japanese eel (Anguilla japonica). Amino acid homology of the open reading frames between alpha and beta in AR or PR is approximately 40%, but the DNA- and ligand-binding domains show high homology between subtypes. Judging from these structures, alpha and beta are not isoforms derived from translational initiation at two in-phase ATG codons, alternative splicing, or tetraploidy. In transient transfection assays using a reporter construct containing a steroid-responsive promoter, each subtype showed its corresponding hormone-dependent transactivation. The ligand affinity for transactivation between AR and PR subtypes was similar for physiological ligands. Tissue distribution of both subtype mRNAs was different. Protein interaction between subtypes was demonstrated in vitro by GST pull-downassays. These results clearly indicate that two functional subtypes of AR and PR exist in eel. These findings will advance our understanding of the mechanisms underlying sex steroid signaling. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:51:33