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Titolo:
Multiple responses in gene expression in fish treated with estrogen
Autore:
Denslow, ND; Lee, HS; Bowman, CJ; Hemmer, MJ; Folmar, LC;
Indirizzi:
Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 Mol Biol, Gainesville, FL 32610 USA Univ Florida, Biotechnol Program, Gainesville, FL 32611 USA Univ Florida Gainesville FL USA 32611 Program, Gainesville, FL 32611 USA Univ Florida, Grad Program Physiol & Pharmacol, Gainesville, FL 32611 USA Univ Florida Gainesville FL USA 32611 harmacol, Gainesville, FL 32611 USA USEPA, Gulf Breeze, FL USA USEPA Gulf Breeze FL USAUSEPA, Gulf Breeze, FL USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 2-3, volume: 129, anno: 2001,
pagine: 277 - 282
SICI:
1096-4959(200106)129:2-3<277:MRIGEI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAINBOW-TROUT; ONCORHYNCHUS-MYKISS; RECEPTOR-ALPHA; IN-VITRO; VITELLOGENIN; ACTIVATION; BETA; REPRODUCTION; TESTOSTERONE; ESTRADIOL;
Keywords:
vitellogenin; estrogen receptor; estradiol; endocrine disrupters; mRNA; differential display PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Denslow, ND Univ Florida, Dept Biochem & Mol Biol, POB 100156 HC, Gainesville, FL 32610 USA Univ Florida POB 100156 HC Gainesville FL USA 32610 32610 USA
Citazione:
N.D. Denslow et al., "Multiple responses in gene expression in fish treated with estrogen", COMP BIOC B, 129(2-3), 2001, pp. 277-282

Abstract

During the last decade there has been a significant body of research conducted on environmental estrogens. These, include industrial, agricultural and pest-control chemicals that bind to the estrogen receptor and induce biological changes during development or reproduction. Most of these changes are probably due to modified gene expression, since estrogen receptors function at this level. We have mapped qualitative gene expression responses (by differential display reverse transcriptase polymerase chain reaction, DD) in adult male sheepshead minnows (Cyprinidon variegatus) receiving high doseinjections (5 mg/kg), or constant flow-through aquatic exposures to environmentally relevant concentrations (100 ng/l) of estradiol-17 beta, and found them nearly identical. We have observed both up-regulation and down-regulation of transcripts, which fit into known responses to estradiol. Among the genes up-regulated are vitellogenin and several vitelline envelope proteins indicating that genes for proteins involved in egg development and maturation are susceptible to environmental estrogen exposure. While physiological changes caused by estradiol treatment are not totally explained by changes at the mRNA level, those changes can nevertheless be used as fingerprints to characterize an in vivo estrogenic response. (C) 2001 Elsevier ScienceInc. Ah rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:20:02