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Titolo:
Fez1/Lzts1 alterations in gastric carcinoma
Autore:
Vecchione, A; Ishii, H; Shiao, YH; Trapasso, F; Rugge, M; Tamburrino, JF; Murakumo, Y; Alder, H; Croce, CM; Baffa, R;
Indirizzi:
Thomas Jefferson Univ, Jefferson Med Coll, Kimmel Canc Ctr, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA NCI, Frederick Canc Res & Dev Ctr, Comparat Carcinogenesis Lab, Frederick,MD 21702 USA NCI Frederick MD USA 21702 rat Carcinogenesis Lab, Frederick,MD 21702 USA Univ Padua, Dept Pathol, I-35126 Padua, Italy Univ Padua Padua Italy I-35126 Padua, Dept Pathol, I-35126 Padua, Italy
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 6, volume: 7, anno: 2001,
pagine: 1546 - 1552
SICI:
1078-0432(200106)7:6<1546:FAIGC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOCARCINOMA; CANCER; HETEROZYGOSITY; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Baffa, R Thomas Jefferson Univ, Jefferson Med Coll, Kimmel Canc Ctr, 1015 Walnut St,Suite 1102A, Philadelphia, PA 19107 USA Thomas Jefferson Univ 1015 Walnut St,Suite 1102A Philadelphia PA USA 19107
Citazione:
A. Vecchione et al., "Fez1/Lzts1 alterations in gastric carcinoma", CLIN CANC R, 7(6), 2001, pp. 1546-1552

Abstract

Purpose: Loss of heterozygosity (LOH) involving the short arm of chromosome 8 (8p) is a common feature of the malignant progression of human tumors, including gastric cancer, We have cloned and mapped a candidate tumor suppressor gene, FEZ1/LZTS1, to 8p22, Here we have analyzed whether FEZ1/LZTS1 alterations play a role in the development and progression of gastric carcinoma. Experimental Design: We examined Fez1/LZTS1 expression in 8 gastric carcinoma cell lines by Western blot, and in 88 primary gastric carcinomas by immunohistochemistry. Twenty-six of these 88 primary gastric carcinomas were also microdissected and tested for LOH at the FEZ1/LZTS1 locus and for mutation of the FEZI/LZTSI gene. Furthermore, we studied the FEZ1/LZTS1 gene regulation and transcriptional control and the methylation status of the 5' region of the gem in ah 8 gastric carcinoma cell lines. Results: Fez1/Lzts1 protein was barely detectable in all of the gastric cancer cell lines tested and was absent or significantly reduced in 39 of the88 (44.3%) gastric tarcinomas analyzed by immunohistochemistry,,vith a significant correlation (P < 0.001) to diffuse histotype, DNA allelotyping analysis showed allelic loss in 3 of 17 (18%) and microsatellite instability in 4 of 17 (23.5%) cases informative for D8S261 at the FEZ1/LZTS1 locus. When we compared the presence of LOH with Fez1/Lzts1 expression, we found lossof protein expression in all three of the tumors with allelic imbalance atD8S261, A missense mutation was detected in one case that did not express Fez1/Lzts1, Hypermethylation of the CpG island flanking the Fez1/Lzts1 promoter was evident in six of the eight tell lines examined as well as in the normal control. Conclusions: Our findings support FEZI/LZTSI as a candidate tumor suppressor gene at gp in a subtype of gastric cancer and suggest that its inactivation is attributable to several factors including genomic deletion and methylation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:52:31