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Titolo:
Molecular determination of perivesical and lymph node metastasis after radical cystectomy for urothelial carcinoma of the bladder
Autore:
Seraj, MJ; Thomas, AR; Chin, JL; Theodorescu, D;
Indirizzi:
Univ Virginia, Hlth Sci Ctr, Dept Urol, Charlottesville, VA 22908 USA UnivVirginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Hlth Sci Ctr, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Western Ontario, Dept Surg, Div Urol, London, ON N6A 3K7, Canada UnivWestern Ontario London ON Canada N6A 3K7 London, ON N6A 3K7, Canada
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 6, volume: 7, anno: 2001,
pagine: 1516 - 1522
SICI:
1078-0432(200106)7:6<1516:MDOPAL>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSITIONAL-CELL CARCINOMA; CIRCULATING CANCER-CELLS; PHASE-II TRIAL; UROPLAKIN-II; PERIPHERAL-BLOOD; VINBLASTINE; CISPLATIN; SURVIVAL; METHOTREXATE; DOXORUBICIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Theodorescu, D Univ Virginia, Hlth Sci Ctr, Dept Urol, Box 422, Charlottesville, VA 22908USA Univ Virginia Box 422 Charlottesville VA USA 22908 22908USA
Citazione:
M.J. Seraj et al., "Molecular determination of perivesical and lymph node metastasis after radical cystectomy for urothelial carcinoma of the bladder", CLIN CANC R, 7(6), 2001, pp. 1516-1522

Abstract

Purpose: Current methods used to determine the pathological stage of the primary tumor and associated lymphatics after radical cystectomy are tedious, costly, and may lack the sensitivity afforded by molecular approaches such as reverse transcription-PCR (RT-PCR) for markers specific for urothelialtissue such as the uroplakin II (UPII) gene. Thus, we sought to evaluate an objective and sensitive molecular approach for the assessment of perivesical extension and lymph node status after radical cystectomy, based on the detection of UPII expression using RT-PCR and compare this assay to standard clinical and pathological examination,Experimental Design: From November 1999 to September 2000, 27 patients with clinical T-a-T3N0M0 urothelial bladder cancer underwent radical cystectomy, 19 (70%) of which also had pelvic lymphadenectomy. At the completion of cystectomy, systematic biopsies of the external surface of the bladder specimen as well as from the largest palpable lymph node found at lymphadenectomy were obtained for molecular analysis, RT-PCR analysis for UPII mRNA was carried out on these biopsy specimens, and results were compared with data obtained from conventional pathological examination. Results: Pathologically organ-confined tumors had a 42% (5 of 12) incidence of positive signals in the perivesical tissues and 17% (1 of 7) in the lymph nodes, Corresponding percentages for pT(3a)N(0) and pT(3b)-T4N0 lesionswere 67% (4 of 6)/25% (1 of 4) and 67% (4 of 6)/33% (2 of 6), respectively. Overall, pathologcally node-negative cancers had a perivesical positivityrate of 54% (13 of 24) and a lymph node positivity rate of 25% (4 of 16), All patients with pathologically positive nodes had positive UPII signals in the lymph node sample,Conclusions: This molecular assay aimed at assessing perivesical extensionand lymph node status after radical cystectomy appears to identify patients that may harbor residual disease not appreciated by conventional histology, Larger studies with 5-7-year follow-up will be required to determine theprognostic significance of such molecular information.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:38:05